Dental pathogens lead to chronic diseases like periodontitis that causes loss of teeth. This research was to examine the plausible antibacterial efficacy of copper nanoparticles (CME-CuNPs) synthesized using Cupressus macrocarpa extract (CME) against periodontitis-causing bacteria. Moreover, antimicrobial properties of CME-CuNPs were assessed against oral microbes(M. luteus. B. subtilis, P. aerioginosa), which causes periodontal disease which was identified using morphological, biochemical analysis, and 16S-rRNA techniques. The CME-CuNPs were characterized by analysis techniques. Accordingly, the peak found at 577 nm using UV-vis spectrometer explained the formation of stable CME-CuNPs. Also, the results revealed the formation of spherical and oblong monodispersed CME-CuNPs with sizes ranged from 11.3 to 22.4 nm. The FTIR analysis suggested that the CME contains reducing agents. Consequently, they had a role in Cu reduction, and CME-CuNPs were formed. Furthermore, the CME-CuNPs exhibited potent antimicrobial efficacy against different isolates and it was superior to the reported values in literature. Antibacterial efficacy of CME-CuNPs upon testing bacteriawas compared to the synergistic solutionof clindamycin with CME-CuNPs. Particularly, synergistic solutions exhibited a superior capacity to prevent the selected bacterialgrowth. Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC), and FIC (Fractional Inhibitory Concentration) of CME-CuNPs with clindamycin recorded against the selected periodontal disease-causing microorganisms are observed between the range of 2.6-3.6 μg/ml, 4-5 μg/ml and 0.312-0.5, respectively. Finally, we employed different testedstrains to elucidate the synergistic effect of CME-CuNPs and clindamycin and the antimicrobial efficacy exhibited by CME-CuNPs with clindamycin could be useful for the future development of more effective treatments for the control of dental diseases.

中文翻译：

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铜纳米药物的绿色合成及其对牙周致病微生物的牙科应用。

牙科病原体会导致慢性疾病，例如导致牙齿脱落的牙周炎。本研究旨在检验使用柏木提取物 (CME) 合成的铜纳米粒子 (CME-CuNPs) 对牙周炎致病菌的合理抗菌功效。此外，还评估了 CME-CuNPs 对口腔微生物（M. luteus、B. subtilis、P. aerioginosa ）的抗菌性能)，导致牙周病，使用形态学、生化分析和 16S-rRNA 技术鉴定。CME-CuNPs 通过分析技术进行了表征。因此，使用紫外-可见光谱仪在 577 nm 处发现的峰解释了稳定 CME-CuNP 的形成。此外，结果显示形成了球形和椭圆形单分散 CME-CuNP，尺寸范围为 11.3 至 22.4 nm。FTIR 分析表明 CME 含有还原剂。因此，它们在 Cu 还原中发挥作用，并形成 CME-CuNP。此外，CME-CuNPs 对不同分离株表现出强大的抗菌功效，优于文献报道的值。将 CME-CuNPs 在测试细菌时的抗菌效果与克林霉素与 CME-CuNPs 的协同溶液进行比较。特别，协同解决方案显示出防止所选细菌生长的卓越能力。观察到 CME-CuNPs 与克林霉素对所选牙周病致病微生物的最低抑菌浓度 (MIC)、最低杀菌浓度 (MBC) 和 FIC（部分抑制浓度）在 2.6-3.6 μg/ml 的范围内，4 -5 微克/毫升和 0.312-0.5，分别。最后，我们采用不同的测试菌株来阐明 CME-CuNPs 和克林霉素的协同作用，CME-CuNPs 与克林霉素表现出的抗菌功效可用于未来开发更有效的牙科疾病控制治疗方法。CME-CuNPs 与克林霉素的最低杀菌浓度 (MBC) 和 FIC（部分抑制浓度）记录在 2.6-3.6 μg/ml、4-5 μg/ml 和 0.312 之间-0.5，分别。最后，我们采用不同的测试菌株来阐明 CME-CuNPs 和克林霉素的协同作用，CME-CuNPs 与克林霉素表现出的抗菌功效可用于未来开发更有效的牙科疾病控制治疗方法。CME-CuNPs 与克林霉素的最低杀菌浓度 (MBC) 和 FIC（部分抑制浓度）记录在 2.6-3.6 μg/ml、4-5 μg/ml 和 0.312 之间-0.5，分别。最后，我们采用不同的测试菌株来阐明 CME-CuNPs 和克林霉素的协同作用，CME-CuNPs 与克林霉素表现出的抗菌功效可用于未来开发更有效的牙科疾病控制治疗方法。