It was reported that Lagerstroemia ovalifolia Teijsm. & Binn. (LO) has been used as Traditional herbal medicine (THM) in Java. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. Collectively, the aforementioned findings indicated that LOLE exerted an ameliorative effect on inflammatory response in airway of ALI mice.

中文翻译：

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卵叶紫薇通过下调 MAPK 和 NF-κB 激活对 LPS 诱导的 ALI 小鼠发挥抗炎作用。

据报道，卵叶紫薇Teijsm。&宾恩。(LO) 在爪哇已被用作传统草药 (THM)。我们之前的研究表明，LO 叶提取物 (LOLE) 对脂多糖 (LPS) 刺激的 RAW264.7 巨噬细胞具有抗炎作用。基于这一发现，当前的研究旨在评估 LOLE 在 LPS 诱导的急性肺损伤 (ALI) 小鼠模型中的保护作用。结果表明，LPS 处理增强了炎症细胞向肺部的流入，增加了小鼠支气管肺泡灌洗液 (BALF) 中巨噬细胞的数量和炎症细胞因子的分泌。然而，这些影响被 LOLE 预处理显着消除。此外，诱导型一氧化氮合酶 (iNOS) 的增加，LOLE 还可以逆转 ALI 小鼠肺组织中环氧合酶 2 (COX-2) 和单核细胞趋化蛋白 1 (MCP-1) 的表达。此外，LOLE 显着抑制 LPS 诱导的 MAPK/NF-κB 信号通路的激活，并导致肺部血红素加氧酶 1 (HO-1) 的诱导。此外，体外实验表明，LOLE可增强RAW264.7巨噬细胞中HO-1的表达。总的来说，上述发现表明 LOLE 对 ALI 小鼠气道炎症反应具有改善作用。