Introduction: LncRNA rhabdomyosarcoma 2-associated transcript (RMST) serves as a key regulator in neural stem cell fate and is involved in the progression of different neurological diseases. In this research, the serum level and clinical value of RMST in Parkinson’s disease (PD) patients were detected, and the underlying mechanism was explored. Methods: Ninety-nine PD patients and 93 healthy individuals were collected for clinical experiments. SH-SY5Y cells were treated with the dopaminergic neurotoxin 1-methyl-4-phenylpyridinium (MPP⁺) to establish PD cell models. qRT-PCR was used for the detection of mRNA levels. CCK-8 and flow cytometry were used to detect neuronal viability and apoptosis. The target relationship of RMST with miR-15a-5p was confirmed applying luciferase reporter assay. Results: RMST was present at high levels in both serum of PD patients and PD cell models. Serum RMST had a certain clinical value for the diagnosis of PD with the AUC of 0.892 at a cutoff value of 1.225. Serum RMST was positively associated with the levels of TNF-α (r = 0.421, p #x3c; 0.001) and IL-1β (r = 0.567, p #x3c; 0.001) in PD patients. Knockdown of RMST alleviated the apoptosis and inflammatory response of SH-SY5Y cells induced by MPP⁺. miR-150-5p was the target gene of RMST and less expressed in the clinical serum samples and PD cell models. Conclusion: Serum RMST serves as a promising biomarker for the diagnosis of PD. RMST downregulation may regulate the occurrence and development of PD through inhibiting neuron cell apoptosis and the release of inflammatory cytokines via targeting miR-150-5p.
Neuroimmunomodulation

中文翻译：

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LncRNA RMST 通过海绵化 miR-150-5p 在帕金森病中调节神经元凋亡和炎症反应

简介： LncRNA 横纹肌肉瘤 2 相关转录本 (RMST) 是神经干细胞命运的关键调节因子，并参与不同神经系统疾病的进展。本研究检测帕金森病（PD）患者RMST的血清水平及临床价值，并探讨其潜在机制。方法：收集99名PD患者和93名健康人进行临床实验。SH-SY5Y 细胞用多巴胺能神经毒素 1-甲基-4-苯基吡啶鎓 (MPP ⁺) 建立 PD 细胞模型。qRT-PCR用于检测mRNA水平。CCK-8 和流式细胞术用于检测神经元活力和细胞凋亡。RMST 与 miR-15a-5p 的靶点关系通过荧光素酶报告基因检测得到证实。结果： RMST 在 PD 患者的血清和 PD 细胞模型中均以高水平存在。血清RMST对PD的诊断具有一定的临床价值，AUC为0.892，截断值为1.225。血清 RMST 与PD 患者的 TNF-α ( r = 0.421, p #x3c; 0.001) 和 IL-1β ( r = 0.567, p #x3c; 0.001) 水平呈正相关。敲低 RMST 可减轻 MPP 诱导的 SH-SY5Y 细胞的凋亡和炎症反应⁺ . miR-150-5p是RMST的靶基因，在临床血清样本和PD细胞模型中表达较少。结论：血清 RMST 可作为诊断 PD 的有希望的生物标志物。RMST下调可能通过靶向miR-150-5p抑制神经元细胞凋亡和炎性细胞因子的释放来调节PD的发生和发展。
神经免疫调节