Detection of chemical warfare agents (CWA) by simple and rapid methods with real-sample applications are quite inevitable in order to ease the threats to living systems caused by uncertain terror attacks and wars. Herein we have developed the first far-red to near infra-red (NIR) probe based on a covalent assembly approach for the detection of trace amounts of nerve agent mimic diethyl chloro phosphate (DCP) in soil and their fluorescent bio imaging in live cells. The probe features abrupt fluorescence turn on sensing of DCP with fluorescence quantum yield Φ = 0.622. It senses DCP selectively over other analytes in excellent sensitivity with a detection limit of 6.9 nM. In real time, the probe treated strips were employed to detect the DCP vapor effectively with eye catching fluorescence response. The presence of trace amounts of these acute warfare agents in the environment were monitored by soil analysis. Further fluorescent bio imaging was carried out to monitor trace level DCP in living cells using the HeLa cell line.

中文翻译：

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“共价组装”——触发远红至近红外发射荧光探针，用于突然检测神经剂模拟物 (DCP)：实时应用监测土壤和活细胞中痕量的存在

为了缓解不确定的恐怖袭击和战争对生命系统造成的威胁，通过简单快速的方法和真实样本应用来检测化学战剂 (CWA) 是不可避免的。在这里，我们开发了第一个基于共价组装方法的远红外到近红外 (NIR) 探针，用于检测土壤中的痕量神经毒剂模拟氯磷酸二乙酯 (DCP) 及其在活细胞中的荧光生物成像. 该探针具有对 DCP 的突然荧光开启传感，荧光量子产率 Φ = 0.622。它以出色的灵敏度选择性地检测 DCP，而不是其他分析物，检测限为 6.9 nM。实时地，探针处理的条带用于有效地检测 DCP 蒸汽，具有引人注目的荧光响应。通过土壤分析监测环境中这些急性战剂的微量存在。使用 HeLa 细胞系进行进一步的荧光生物成像以监测活细胞中的痕量 DCP。