Sortase A, a transpeptidase enzyme is present in many Gram-positive bacteria and helps in the recruitment of the cell surface proteins. Over the last two decades, Sortase A has become an attractive tool for performing in vivo and in vitro ligations. Sortase A-mediated ligation has continuously been used for its specificity, robustness, and highly efficient nature. These properties make it a popular choice among protein engineers as well as researchers from different fields. In this review, we give an overview of Sortase A-mediated ligation of various molecules on the cell surfaces, which can have diverse applications in interdisciplinary fields.

中文翻译：

---

Sortase A：一种用于标记细胞表面的化学酶方法

Sortase A 是一种转肽酶，存在于许多革兰氏阳性细菌中，有助于募集细胞表面蛋白。在过去的二十年里，Sortase A 已成为进行体内和体外结扎的有吸引力的工具。Sortase A 介导的连接因其特异性、稳健性和高效性而不断被使用。这些特性使其成为蛋白质工程师和不同领域研究人员的热门选择。在这篇综述中，我们概述了 Sortase A 介导的各种分子在细胞表面的连接，它们可以在跨学科领域具有多种应用。