Stable and positive social bonds are pretty vital to the development of animals. Instability and disruptions of social bonds, such as maternal separation and social isolation, always produce disastrous influence on physiology, neuroendocrine and behaviors. Pair bond is one of the most important social bonds in adulthood. But the different effects of pair bond interruption between males and females are rarely studied. In the present study, the monogamous mandarin voles (Microtus mandarinus) were used to confirm the time window of pair bond formation. After that, voles were separated from their partner for 1 or 2 weeks. Then anxiety- and depression-like behaviors were investigated by using open field test, light-dark box test, tail suspension test and forced swimming test, respectively. The results showed that: (1) cohabitation for 5 days is sufficient and necessary for mandarin voles to form pair bond; (2) loss of partner is always crucial for the effects of pair bond interruption, while social isolation works in certain behavioral tests.; (3) pair bond interruption for 2 weeks significantly increased the level of anxiety and depression in adult males, but not female mandarin voles. Overall, this research suggested that loss of partner plays a key role in pair bond interruption in male mandarin voles.

稳定和积极的社会纽带对动物的发展非常重要。社会纽带的不稳定和破坏，例如母性分离和社会孤立，总是会对生理、神经内分泌和行为产生灾难性的影响。配对纽带是成年期最重要的社会纽带之一。但是很少研究男性和女性之间配对键中断的不同影响。在本研究中，一夫一妻制的普通话田鼠（Microtus mandarinus) 用于确认对键形成的时间窗口。之后，田鼠与它们的伴侣分开了 1 或 2 周。然后分别采用旷场试验、明暗箱试验、悬尾试验和强迫游泳试验研究焦虑样和抑郁样行为。结果表明：（1）同居5天是田鼠形成对偶的充分必要条件；(2) 失去伴侣对于伴侣关系中断的影响总是至关重要的，而社会隔离在某些行为测试中起作用。(3) 对键中断2周显着增加了成年雄性田鼠的焦虑和抑郁程度，但对雌性田鼠没有影响。总体而言，这项研究表明，伴侣的丧失在雄性柑橘田鼠的配对中断中起着关键作用。