Abstract

Purpose

This study was aimed at investigating the protective effect of antioxidant-rich fraction of Croton zambsicus (C-ZAMB) leaves on ocular-gastrointestinal dysfunction in rats exposed to environmental mixed-metal (EOMABRSL).

Materials and Methods

The rats were divided into five (n = 10) groups. Group I designates the control which received 0.5 mL of distilled water. Group II and III received 0.5 mL of EOMABRSL for 98 days (non-withdrawal) and 70 days (withdrawal for 28 days), respectively. Group IV received 0.5 mL EOMABRSL for 70 days and 400 mg/kg C-ZAMB fraction for 28 days. Group V received 400 mg/kg C-ZAMB only for 28 days via oral route.

Results

Exposure of the animals to EOMARBSL for 98 days and 70 days significantly up-regulated the activities of ocular-gastrointestinal aldolase-reductase, α-amylase, α-glucosidase and eco-5¹-nucleotidase with corresponding depletion of lactate dehydrogenase activity. Furthermore, exposure to EOMABRSL significantly altered the antioxidant proteins with up-production of MDA content. Apparently, management with 400 mg/kg C-ZAMB fraction significantly inhibited the key markers linked with ocular-gastrointestinal disorders.

Conclusion

Hence, this study underscores the biochemical mechanisms for managing ocular-gastrointestinal lesions by 400 mg/kg C-ZAMB fraction on exposure to mixture of environmental metals.

中文翻译：

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在暴露于慢性混合金属的大鼠模型中，富含酚类成分的巴豆叶对眼部和胃肠道功能障碍的元抑制作用

摘要

目的

本研究旨在研究富含抗氧化剂的巴豆（C-ZAMB）叶对暴露于环境混合金属（EOMABRSL）的大鼠眼-胃肠功能障碍的保护作用。

材料和方法

将大鼠分为五组（n  = 10）。第 I 组指定接受 0.5 mL 蒸馏水的对照组。II 组和 III 组分别接受 0.5 mL EOMABRSL 98 天（非停药）和 70 天（停药 28 天）。IV 组接受 0.5 mL EOMABRSL 70 天和 400 mg/kg C-ZAMB 级分 28 天。第 V 组通过口服途径仅接受 400 mg/kg C-ZAMB 28 天。

结果

动物暴露于 EOMARBSL 98 天和 70 天显着上调了眼-胃肠道醛缩酶还原酶、α-淀粉酶、α-葡萄糖苷酶和 eco-5 ¹ -核苷酸酶的活性，同时乳酸脱氢酶活性相应降低。此外，暴露于 EOMABRSL 显着改变了抗氧化蛋白，增加了 MDA 含量。显然，使用 400 mg/kg C-ZAMB 级分进行管理显着抑制了与眼-胃肠道疾病相关的关键标志物。

结论

因此，本研究强调了在暴露于环境金属混合物时通过 400 mg/kg C-ZAMB 组分管理眼-胃肠道病变的生化机制。