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MiR-125b regulates the differentiation of hair follicles in Fine-wool Sheep and Cashmere goats by targeting MXD4 and FGFR2
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-09-06 , DOI: 10.1080/10495398.2021.1968884
Haie Qu 1, 2 , Sufang Wu 1 , Jianping Li 3 , Tao Ma 1 , Jianyu Li 1 , Ba Xiang 1 , Huaizhi Jiang 4 , Qiaoling Zhang 1
Affiliation  

Abstract

With the development of miRNAs identification technology, more and more miRNAs have been discovered, and the role of miRNAs in the development of animal hair follicles has become a focus of research on hair-producing animals. In the previous experiment, compare the microRNA (miRNA) trancriptomes of goats and sheep skin using Solexa sequencing and differentially expressed miR-125b was screened. However, the mechanism of miR-125b regulating hair follicle development is not clear. Therefore, in the present study, the expression of miR-125b, MXD4 and FGFR2 in skin tissue of Fine-wool Sheep and Cashmere goats and HEK-293T cells was examined by qPCR and Western blot. Furthermore, the correlation between miR-125b and the predicted target gene (MXD4, FGFR2) was verified using the Dual-luciferase Reporter assay. We demonstrated that the expression of MXD4 and FGFR2 in Cashmere goats was significantly higher than that of Fine-wool Sheep, and the expression was opposite to that of miR-125b. miR-125b can down-regulate the levels of MXD4 and FGFR2. Dual-luciferase reporter gene assay showed that miR-125b could bind to the 3′-UTR region of target genes FGFR2 and MXD4, suggesting that MXD4 and FGFR2 were target genes of miR-125b. This study has shown that the growth and development of hair follicles in skin tissue of Fine-wool Sheep and Cashmere goats from the new regulatory levels of miRNAs, and clarified the mechanism of miR-125b and its target genes in the development of hair follicles in the skin.



中文翻译:

MiR-125b 通过靶向 MXD4 和 FGFR2 调控细毛绵羊和绒山羊毛囊的分化

摘要

随着miRNAs鉴定技术的发展,越来越多的miRNAs被发现,miRNAs在动物毛囊发育中的作用成为毛发动物研究的热点。在之前的实验中,使用 Solexa 测序比较了山羊和绵羊皮肤的 microRNA (miRNA) 转录组,并筛选了差异表达的 miR-125b。然而,miR-125b调控毛囊发育的机制尚不清楚。因此,在本研究中,通过 qPCR 和 Western blot 检测细毛绵羊和绒山羊皮肤组织和 HEK-293T 细胞中 miR-125b、MXD4 和 FGFR2 的表达。此外,miR-125b 与预测的靶基因(MXD4、FGFR2)之间的相关性通过双荧光素酶报告基因检测得到验证。我们证明MXD4和FGFR2在绒山羊中的表达显着高于细毛绵羊,与miR-125b的表达相反。miR-125b 可以下调 MXD4 和 FGFR2 的水平。双荧光素酶报告基因检测显示miR-125b可以结合靶基因FGFR2和MXD4的3'-UTR区域,提示MXD4和FGFR2是miR-125b的靶基因。本研究从新调控水平的miRNAs揭示了细毛绵羊和绒山羊皮肤组织毛囊的生长发育,阐明了miR-125b及其靶基因在毛囊发育中的作用机制。皮。miR-125b 可以下调 MXD4 和 FGFR2 的水平。双荧光素酶报告基因检测显示miR-125b可以结合靶基因FGFR2和MXD4的3'-UTR区域,提示MXD4和FGFR2是miR-125b的靶基因。本研究从新调控水平的miRNAs揭示了细毛绵羊和绒山羊皮肤组织毛囊的生长发育,阐明了miR-125b及其靶基因在毛囊发育中的作用机制。皮。miR-125b 可以下调 MXD4 和 FGFR2 的水平。双荧光素酶报告基因检测显示miR-125b可以结合靶基因FGFR2和MXD4的3'-UTR区域,提示MXD4和FGFR2是miR-125b的靶基因。本研究从新调控水平的miRNAs揭示了细毛绵羊和绒山羊皮肤组织毛囊的生长发育,阐明了miR-125b及其靶基因在毛囊发育中的作用机制。皮。

更新日期:2021-09-06
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