Abstract

Paint sludge is considered hazardous waste due to its heavy metals and high organic compounds. Incineration and landfilling are the methods of disposing of sludge collected from the paint spray of an automotive factory in Iran. Pseudomonas aeruginosa was isolated from water-based paint sludge using Bushnell-Haas medium and identified by chemical and molecular methods. A two-step bioleaching experiment using the Response surface methodology was used to investigated P. aeruginosa to mobilize the contaminant zinc metal from the paint sludge. Variables included temperatures from 32 to 37 °C, the rotation speed of shaker from 120 to 180 rpm, particle sizes from 1 to 3 mm, and bacteria were adapted to a pulp density of 7 g/L. When the bioleaching process was optimized at pH 7, temperature 32 °C, particle size 3 mm, and 180 rpm, the concentration of zinc decreased to 28.71%. A decrease in zinc of 27% was confirmed by repeating the experiment three times. The ANOVA test confirmed the most effective parameters were temperature and particle size. The most efficient process to remove zinc was achieved as a result of the effect between temperature and pH.

摘要

由于其重金属和高有机化合物，油漆污泥被认为是危险废物。焚烧和填埋是处理伊朗一家汽车厂喷漆中收集的污泥的方法。使用 Bushnell-Haas 介质从水性涂料污泥中分离出铜绿假单胞菌，并通过化学和分子方法进行鉴定。使用响应面方法的两步生物浸出实验用于研究铜绿假单胞菌从油漆污泥中调动污染物锌金属。变量包括从 32 到 37 °C 的温度、从 120 到 180 rpm 的振动器转速、从 1 到 3 mm 的颗粒大小以及使细菌适应 7 g/L 的纸浆密度。当在 pH 7、温度 32°C、粒径 3 mm 和 180 rpm 条件下优化生物浸出工艺时，锌的浓度降低到 28.71%。通过重复实验 3 次，确认锌减少了 27%。ANOVA 测试证实最有效的参数是温度和粒度。由于温度和 pH 值之间的影响，实现了去除锌的最有效过程。