ROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests for RVA detection. All the steps are carried out at room temperature, bringing results in less than two hours. This assay, named ROTADIAL, was validated with a reference panel of feces from pediatric patients from Argentina. The overall sensitivity and specificity of the ROTADIAL test, when compared to a commercial test, was 100 % (100/100) and 99 % (99/100) respectively. ROTADIAL presented optimal analytical performance, being capable of detecting RVA regardless of the presence of other common human enteric infectious agents and is the first RVA-diagnostic assay developed using Nbs, worldwide.

ROTADIAL 是一种基于纳米抗体 (Nb) 的快速 ELISA 检测方法，能够识别儿科患者粪便中的 A 型轮状病毒 (RVA)。该测定基于两个专利的美洲驼衍生 Nbs 的三明治，这些 Nbs 指向来自 RVA 的内部衣壳病毒蛋白 VP6。Nbs 针对 VP6 的构象表位并识别所有测试的人类 RVA 菌株，代表了用于 RVA 检测的免疫诊断测试的理想试剂。所有步骤均在室温下进行，不到两小时即可获得结果。这种名为 ROTADIAL 的检测方法已通过阿根廷儿科患者的粪便参考小组进行了验证。与商业测试相比，ROTADIAL 测试的总体敏感性和特异性分别为 100 % (100/100) 和 99 % (99/100)。ROTADIAL 呈现出最佳的分析性能，