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Differential Expression of Long Noncoding RNAs Reveals a Potential Biomarker for Intractable Pemphigus
Disease Markers ( IF 3.464 ) Pub Date : 2021-09-07 , DOI: 10.1155/2021/5594659
Peng Qu 1, 2 , Zixuan Huang 1 , Haiqin Zhu 1 , Jie Zheng 1 , Meng Pan 1
Affiliation  

Background. Long noncoding RNAs (lncRNAs) are involved in autoimmune diseases. However, the role of lncRNAs in pemphigus remains elusive. Objective. The study is aimed at investigating the expression profile in pemphigus patients to identify a circulating lncRNA as a novel biomarker for pemphigus. Method. A global lncRNA expression profile in peripheral blood mononuclear cells (PBMCs) was measured by lncRNA microarray. Differentially expressed lncRNAs were validated by quantitative reverse transcriptase-PCR (qRT-PCR). The functional and biological processes of the differentially expressed lncRNAs were analyzed by bioinformatics. Results. lncRNA ENST00000585297 in the PBMCs of pemphigus patients was highly expressed compared with those of HCs and BP patients. The area under the receiver operating characteristic (ROC) curve was 0.846 ( to 0.9397). Intriguingly, we found that the expression of ENST00000585297 was upregulated in pemphigus patients whose symptoms could not be managed within four weeks compared to other patients whose symptoms could be managed in four weeks or less (). In addition, ENST00000585297 expression in pemphigus patients was positively correlated with the dosage of prednisone needed to manage the disorder (, ). A competing endogenous RNA (ceRNA) regulatory network was constructed based on the ceRNA theory. Further verification demonstrated that silencing of ENST00000585297 increased the expression of miR-584-3p. Conclusions. Our study revealed for the first time the expression profile of lncRNAs in pemphigus patients. In addition, our study identified ENST00000585297 as a biomarker and indicator for the intractable course of pemphigus.

中文翻译:

长链非编码 RNA 的差异表达揭示了顽固性天疱疮的潜在生物标志物

背景。长链非编码 RNA (lncRNA) 与自身免疫性疾病有关。然而,lncRNA 在天疱疮中的作用仍然难以捉摸。客观的。该研究旨在调查天疱疮患者的表达谱,以确定循环 lncRNA 作为天疱疮的新型生物标志物。方法。通过 lncRNA 微阵列测量外周血单个核细胞 (PBMC) 中的全局 lncRNA 表达谱。通过定量逆转录酶-PCR (qRT-PCR) 验证差异表达的 lncRNA。通过生物信息学分析差异表达的lncRNA的功能和生物学过程。结果。lncRNA ENST00000585297 在天疱疮患者的 PBMCs 中与 HCs 和 BP 患者相比高表达。受试者工作特征(ROC)曲线下面积为 0.846(至 0.9397)。有趣的是,我们发现ENST00000585297的表达在4周内无法控制的天疱疮患者与其他4周或更短的症状可以控制的患者相比上调。)。此外,ENST00000585297 在天疱疮患者中的表达与控制该疾病所需的泼尼松剂量呈正相关。, )。基于ceRNA理论构建了竞争性内源性RNA(ceRNA)调控网络。进一步的验证表明,ENST00000585297 的沉默增加了 miR-584-3p 的表达。结论。我们的研究首次揭示了 lncRNA 在天疱疮患者中的表达谱。此外,我们的研究将 ENST00000585297 确定为天疱疮难治性病程的生物标志物和指标。
更新日期:2021-09-07
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