Abstract

The complete mitochondrial genome of the Cyprinodontiformes fish Poropanchax normani was studied in this study. The size of the entire mitochondrial genome was 16,878 bp, and the total length of the encoding sequence was 11,435 bp, accounting for 67.75%, encoding 3800 amino acids in total. Genome base compositions were: A was 27.30%, C was 28.30%, G was 16.17%, and T was 28.23%. ND2 and CO1 started with GTG, and other protein-coding genes started with ATG. ND1, ND2, and ND3 ended with TAG, respectively, CO2 and ND4 ended with a single T, and other PCGS ended with TAA. The ribosomal RNA lengths of 12S and 16S were 950 bp and 1690 bp, respectively. The control area (D-loop) was 1194 bp in size and ranged from 15,685 to 16,878 bp. It showed negative GC skew value (–0.2728) and negative AT skewness (–0.0168). Phylogenetic analysis showed that P. normani was most closely related to Xenotoca eiseni. The complete sequence of the mitochondrial genome will provide a new perspective for classification and help to draw a more complete picture of species diversity within the Cyprinodontiformes.

摘要

Cyprinodontiformes 鱼Poropanchax normani的完整线粒体基因组本研究进行了研究。整个线粒体基因组大小为16,878 bp，编码序列总长度为11,435 bp，占67.75%，共编码3800个氨基酸。基因组基础组成为：A为27.30%，C为28.30%，G为16.17%，T为28.23%。ND2 和 CO1 以 GTG 开头，其他蛋白质编码基因以 ATG 开头。ND1、ND2 和 ND3 分别以 TAG 结尾，CO2 和 ND4 以单个 T 结尾，其他 PCGS 以 TAA 结尾。12S和16S的核糖体RNA长度分别为950 bp和1690 bp。控制区（D-loop）大小为 1194 bp，范围为 15,685 至 16,878 bp。它显示负 GC 偏斜值 (–0.2728) 和负 AT 偏斜值 (–0.0168)。系统发育分析表明，P. normani与P. normani 的亲缘关系最密切异种异星菌。线粒体基因组的完整序列将为分类提供新的视角，并有助于更全面地了解鲤形目内的物种多样性。