Abstract

Sustained external supply of oxygen (O₂) to engineered tissue constructs is important for their survival in the body while angiogenesis is taking place. In the recent years, the trend towards the fabrication of various O₂-generating materials that can provide prolonged and controlled O₂ source to the large volume tissue constructs resulted in preventing necrosis associated with the lack of O₂ supply. In this review, we explain different methods employed in the fabrication of O₂-generating materials such as emulsion, microfluidics, solvent casting, freeze drying, electrospraying, gelation, microfluidic and three-dimensional (3D) bioprinting methods. After discussing pros and cons of each method, we review physical, chemical, and biological characterisation techniques used to analyse the resulting product. Finally, the challenges and future directions in the field are discussed.

摘要

在血管生成发生时，向工程组织构建体持续外部供应氧气 (O _{2 ) 对于它们在体内的存活很重要。}近年来，制造各种O ₂生成材料的趋势，这些材料可以为大体积组织构建体提供延长和受控的O _{2源，从而防止与O 2}供应不足相关的坏死。_{在这篇综述中，我们解释了用于制造 O 2}的不同方法- 生成材料，例如乳液、微流体、溶剂浇铸、冷冻干燥、电喷雾、凝胶化、微流体和三维 (3D) 生物打印方法。在讨论了每种方法的优缺点后，我们回顾了用于分析所得产品的物理、化学和生物表征技术。最后，讨论了该领域的挑战和未来方向。