In our paper, the effects of As4S4 treatments on the growth and migration of gastric cancer (GC) cells were explored, and the potential underlying molecular mechanisms were also identified. Cell viability was evaluated by cell counting kit 8 assay. The expression of Ki-67 was examined using immunofluorescence staining. Cell apoptosis was assessed by flow cytometry. The migratory and invasion abilities of cells were determined using Transwell assay. The mRNA and protein levels of related gene were examined by RT-qPCR and western blotting, respectively. CircRNAs chip was performed to identify the differentiated expression of circRNAs in GC cells following the treatment with As4S4. Our results revealed that the proliferation, migration and invasion of GC cells were remarkably suppressed by the treatment with As4S4, while cell apoptosis was promoted. Furthermore, circRNA_ASAP2 was a novel target of As4S4 in GC, and it is involved in As4S4-modulated biological behavior alterations in GC cells. In addition, the activities of the Wnt/β-catenin signaling in GC cells were affected by the overexpression circRNA_ASAP2 and the treatment with As4S4. Moreover, the behavior changes in GC cells caused by the knockdown of circRNA_ASAP2 were reversed by the treatment with Wnt agonist SKL2001. In summary, As4S4 could function as an antitumor agent in GC through regulating the circRNA_ASAP2/Wnt/β-catenin pathway, which in turn influences the growth and metastasis of GC cells.

中文翻译：

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硫化砷通过调节 circRNA_ASAP2/Wnt/β-catenin 通路抑制胃癌进展。

在我们的论文中，探讨了 As4S4 处理对胃癌 (GC) 细胞生长和迁移的影响，并确定了潜在的潜在分子机制。通过细胞计数试剂盒8测定法评估细胞活力。使用免疫荧光染色检查 Ki-67 的表达。通过流式细胞术评估细胞凋亡。使用Transwell实验测定细胞的迁移和侵袭能力。分别采用RT-qPCR和western blotting检测相关基因的mRNA和蛋白水平。进行 CircRNA 芯片以鉴定 As4S4 处理后 GC 细胞中 circRNA 的差异表达。我们的结果表明，As4S4 处理显着抑制 GC 细胞的增殖、迁移和侵袭，同时促进细胞凋亡。此外，circRNA_ASAP2是GC中As4S4的新靶标，它参与As4S4调节的GC细胞的生物学行为改变。此外，GC细胞中Wnt/β-catenin信号传导的活性受到circRNA_ASAP2过表达和As4S4处理的影响。此外，Wnt 激动剂 SKL2001 治疗可逆转 circRNA_ASAP2 敲低引起的 GC 细胞行为变化。综上所述，As4S4可以通过调节circRNA_ASAP2/Wnt/β-catenin通路在GC中发挥抗肿瘤作用，进而影响GC细胞的生长和转移。