Neurogenesis persists in restricted regions of the adult brain, including the subventricular zone (SVZ). Adult neural stem cells (NSCs) in the SVZ proliferate and give rise to new neurons and glial cells depending on intrinsic and environmental cues. Among the multiple factors that contribute to the chemical, physical, and mechanical components of the neurogenic niche, we focused on the composition of the extracellular matrix (ECM) of vasculature and fractones in the SVZ. The SVZ consists of ECM-rich blood vessels and fractones during development and adulthood, and adult neural stem/progenitor cells (NS/PCs) preferentially attach to the laminin-rich basal lamina. To examine the ECM preference of adult NS/PCs, we designed a competition assay using cell micropatterning. Although both laminin and collagen type IV, which are the main components of basal lamina, act as physical scaffolds, adult NS/PCs preferred to adhere to laminin over collagen type IV. Interestingly, the ECM preference of adult NS/PCs could be manipulated by chemokines such as stromal-derived factor 1 (SDF1) and α6 integrin. As SDF1 re-routes NSCs and their progenitors toward the injury site after brain damage, these results suggest that the alteration in ECM preferences may provide a molecular basis for contextdependent NS/PC positioning.

中文翻译：

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成人脑室下区细胞外基质成分景观对神经干/祖细胞定位的贡献。

神经发生持续存在于成人大脑的受限区域，包括脑室下区 (SVZ)。SVZ 中的成体神经干细胞 (NSC) 根据内在和环境线索增殖并产生新的神经元和神经胶质细胞。在影响神经源性生态位的化学、物理和机械成分的多种因素中，我们专注于 SVZ 中脉管系统和 fractones 的细胞外基质 (ECM) 的组成。SVZ 由发育和成年期间富含 ECM 的血管和分枝结构组成，成年神经干/祖细胞 (NS/PC) 优先附着在富含层粘连蛋白的基底层上。为了检查成人 NS/PC 的 ECM 偏好，我们设计了使用细胞微模式的竞争测定。尽管层粘连蛋白和 IV 型胶原蛋白，作为基底层的主要成分，作为物理支架，成人 NS/PCs 更喜欢粘附在层粘连蛋白上而不是 IV 型胶原蛋白。有趣的是，成人 NS/PC 的 ECM 偏好可以通过诸如基质衍生因子 1 (SDF1) 和 α6 整合素等趋化因子来操纵。由于 SDF1 在脑损伤后将 NSC 及其祖细胞重新路由到损伤部位，这些结果表明 ECM 偏好的改变可能为上下文相关的 NS/PC 定位提供了分子基础。