With the attention of biomineralization, calcium phosphate precursor phases regulated by different organic templates are synthesized and used as mineral sources for biomimetic mineralization. However, most of the organic templates have limited ability to stabilize calcium and phosphorus ions, or the selected templates have poor biocompatibility and irremovability, which make them difficult to co-exist in long-term stability and crystal transformation, and to achieve convenient and harmless clinical application. Herein, we propose a calcium phosphate prenucleation clusters (CaP-PNCs)/starch complex, which includes uniform-sized, separate, and homogeneously distributed amorphous calcium phosphate nanoclusters of about 1 nm. By means of freeze-drying and redissolution, the CaP-PNCs/starch complex can be converted between liquid and spongy solid without changing their morphology and amorphous phase, and the freeze-dried CaP-PNCs/starch complex can be stored at room temperature for at least 5 months. Imitating the mechanism of protein-protease controlled biomineralization in organisms, the CaP-PNCs/starch complex is mixed with α-amylase during application. Starch templates are removed by enzymolysis and CaP-PNCs can be released, thus triggering on-site biomimetic mineralization with enamel-like structures to repair tooth tissue defects (including enamel and dentin). CaP-PNCs/starch complex provides a smart mineral source supply for biomimetic mineralization and a new pathway for the design of biomimetic materials.

随着生物矿化的关注，合成了由不同有机模板调控的磷酸钙前体相，并将其用作仿生矿化的矿物来源。然而，大多数有机模板对钙磷离子的稳定能力有限，或者所选模板的生物相容性和不可去除性差，难以长期稳定和结晶转化共存，实现方便无害。临床应用。在此，我们提出了一种磷酸钙预成核簇（CaP-PNCs）/淀粉复合物，它包括大小均匀、分离且均匀分布的约 1 nm 的无定形磷酸钙纳米簇。通过冷冻干燥和再溶解，CaP-PNCs/淀粉复合物可以在液体和海绵状固体之间转换而不改变其形态和无定形相，冻干的CaP-PNCs/淀粉复合物可以在室温下储存至少5个月。模仿生物体内蛋白质-蛋白酶控制生物矿化的机制，CaP-PNCs/淀粉复合物在应用过程中与α-淀粉酶混合。淀粉模板通过酶解被去除，CaP-PNCs 可以被释放，从而触发具有牙釉质结构的现场仿生矿化，以修复牙齿组织缺陷（包括牙釉质和牙本质）。CaP-PNCs/淀粉复合物为仿生矿化提供了智能矿物源供应和仿生材料设计的新途径。冻干的CaP-PNCs/淀粉复合物可在室温下保存至少5个月。模仿生物体内蛋白质-蛋白酶控制生物矿化的机制，CaP-PNCs/淀粉复合物在应用过程中与α-淀粉酶混合。淀粉模板通过酶解被去除，CaP-PNCs 可以被释放，从而触发具有牙釉质结构的现场仿生矿化，以修复牙齿组织缺陷（包括牙釉质和牙本质）。CaP-PNCs/淀粉复合物为仿生矿化提供了智能矿物源供应和仿生材料设计的新途径。冻干的CaP-PNCs/淀粉复合物可在室温下保存至少5个月。模仿生物体内蛋白质-蛋白酶控制生物矿化的机制，CaP-PNCs/淀粉复合物在应用过程中与α-淀粉酶混合。淀粉模板通过酶解被去除，CaP-PNCs 可以被释放，从而触发具有牙釉质结构的现场仿生矿化，以修复牙齿组织缺陷（包括牙釉质和牙本质）。CaP-PNCs/淀粉复合物为仿生矿化提供了智能矿物源供应和仿生材料设计的新途径。淀粉模板通过酶解被去除，CaP-PNCs 可以被释放，从而触发具有牙釉质结构的现场仿生矿化，以修复牙齿组织缺陷（包括牙釉质和牙本质）。CaP-PNCs/淀粉复合物为仿生矿化提供了智能矿物源供应和仿生材料设计的新途径。淀粉模板通过酶解被去除，CaP-PNCs 可以被释放，从而触发具有牙釉质结构的现场仿生矿化，以修复牙齿组织缺陷（包括牙釉质和牙本质）。CaP-PNCs/淀粉复合物为仿生矿化提供了智能矿物源供应和仿生材料设计的新途径。