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Generation of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette
Tropical Plant Biology ( IF 2 ) Pub Date : 2021-09-06 , DOI: 10.1007/s12042-021-09298-z
F. G. Faleiro 1, 2 , J. Y. Kim 1, 3 , A. Parikh 1 , F. Altpeter 1
Affiliation  

Napier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tremendous potential for crop improvement and require the establishment of a transformation protocol. In this study, transgenic Napier grass was generated by introducing a minimal cassette (MC) encoding the antibiotic resistance gene nptII with biolistic gene transfer into embryogenic callus. Paromomycin was superior to geneticin as a selective agent in the selection of transgenic calli. Two high-biomass producing Napier grass accessions were successfully transformed. Southern blot analysis revealed simple transgene integration patterns with 33% of the regenerated transgenic lines having a single insert of the minimal transgene expression cassette. NPTII-ELISA confirmed transgene expression in all transgenic lines. The results of this study will enable the acceleration of Napier grass improvement by genetic transformation or genome editing.



中文翻译:

通过最小表达盒的基因枪基因转移产生转基因纳皮尔草 (Cenchrus purpureus Schum.) 植物

Napier 草 ( Cenchrus purpureus Schum.) 是产量最高的热带 C4 牧草之一,也是生产生物燃料的有前途的候选原料。基因工程和基因组编辑在作物改良方面具有巨大潜力,需要建立转化方案。在这项研究中,转基因纳皮尔草是通过引入编码抗生素抗性基因npt的最小盒 (MC) 产生的II 用基因枪基因转移到胚性愈伤组织中。巴龙霉素作为转基因愈伤组织的选择剂优于遗传霉素。成功转化了两种高生物质纳皮尔草种质。Southern 印迹分析揭示了简单的转基因整合模式,其中 33% 的再生转基因品系具有单个插入的最小转基因表达盒。NPTII-ELISA 证实了所有转基因品系中的转基因表达。这项研究的结果将通过遗传转化或基因组编辑加速纳皮尔草的改良。

更新日期:2021-09-06
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