This paper discusses recovery of erythromycin (EMC) from the solvent rich phase formed after sugaring-out extraction (SOE), a novel two phase separation technique. The conditions for SOE of EMC have already been optimized (glucose 15.6% w/w, temperature 4 °C, pH 8.3, and solvent/broth ratio 1:1). The solvent i.e. acetonitrile (ACN) rich phase was further processed to separate EMC using crystallization. Three different modes of crystallization of EMC were studied for a model system: evaporative, antisolvent (water), and combined antisolvent-evaporative. It was found that EMC degraded during evaporative crystallization, substantially lowering the yield. Antisolvent crystallization at the EMC concentration used did not yield any crystals. The combined antisolvent-evaporative crystallization resulted in the formation of desirable plate like crystals with a yield of 95% and was explored further using simulated fermentation broth (SFB) which resulted in a yield of 92.2% with desired crystal morphology. A mathematical model for EMC crystallization was also developed and the crystallization kinetics was estimated by fitting model predictions with the experimental data. Furthermore, antimicrobial activity of the crystallized EMC was found comparable to the commercial sample, showing that the chosen purification technique did not impact the efficacy of the recovered EMC.

本文讨论了从糖化萃取 (SOE) 后形成的富溶剂相中回收红霉素 (EMC)，这是一种新型的两相分离技术。EMC 的 SOE 条件已经优化（葡萄糖 15.6% w/w，温度 4 °C，pH 8.3，溶剂/肉汤比例 1:1）。溶剂即富含乙腈(ACN)的相被进一步处理以使用结晶分离EMC。针对模型系统研究了三种不同的 EMC 结晶模式：蒸发、反溶剂（水）和组合反溶剂-蒸发。发现在蒸发结晶过程中 EMC 降解，显着降低了产率。在所用 EMC 浓度下的抗溶剂结晶未产生任何晶体。组合的抗溶剂蒸发结晶导致以 95% 的产率形成所需的板状晶体，并使用模拟发酵液 (SFB) 进行进一步探索，产生 92.2% 的产率并具有所需的晶体形态。还开发了 EMC 结晶的数学模型，并通过将模型预测与实验数据拟合来估计结晶动力学。此外，发现结晶 EMC 的抗菌活性与商业样品相当，表明所选的纯化技术不会影响回收的 EMC 的功效。还开发了 EMC 结晶的数学模型，并通过将模型预测与实验数据拟合来估计结晶动力学。此外，发现结晶 EMC 的抗菌活性与商业样品相当，表明所选的纯化技术不会影响回收的 EMC 的功效。还开发了 EMC 结晶的数学模型，并通过将模型预测与实验数据拟合来估计结晶动力学。此外，发现结晶 EMC 的抗菌活性与商业样品相当，表明所选的纯化技术不会影响回收的 EMC 的功效。