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Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions
The ISME Journal ( IF 11.0 ) Pub Date : 2021-09-06 , DOI: 10.1038/s41396-021-01101-x
Kjell Rune Jonassen 1, 2 , Live H Hagen 1 , Silas H W Vick 1 , Magnus Ø Arntzen 1 , Vincent G H Eijsink 1 , Åsa Frostegård 1 , Pawel Lycus 1 , Lars Molstad 3 , Phillip B Pope 1, 4 , Lars R Bakken 1
Affiliation  

Inoculating agricultural soils with nitrous oxide respiring bacteria (NRB) can reduce N2O-emission, but would be impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production are suitable substrates and vectors for NRB. We show that indigenous NRB in digestates grew to high abundance during anaerobic enrichment under N2O. Gas-kinetics and meta-omic analyses showed that these NRB’s, recovered as metagenome-assembled genomes (MAGs), grew by harvesting fermentation intermediates of the methanogenic consortium. Three NRB’s were isolated, one of which matched the recovered MAG of a Dechloromonas, deemed by proteomics to be the dominant producer of N2O-reductase in the enrichment. While the isolates harbored genes required for a full denitrification pathway and could thus both produce and sequester N2O, their regulatory traits predicted that they act as N2O sinks in soil, which was confirmed experimentally. The isolates were grown by aerobic respiration in digestates, and fertilization with these NRB-enriched digestates reduced N2O emissions from soil. Our use of digestates for low-cost and large-scale inoculation with NRB in soil can be taken as a blueprint for future applications of this powerful instrument to engineer the soil microbiome, be it for enhancing plant growth, bioremediation, or any other desirable function.



中文翻译:

沼气消化物中的一氧化二氮呼吸细菌可减少农业排放

用一氧化二氮呼吸细菌 (NRB) 接种农业土壤可以减少 N 2 O 排放,但作为独立操作是不切实际的。在这里,我们证明沼气生产后获得的消化物是 NRB 的合适底物和载体。我们表明,在 N 2 O下的厌氧富集过程中,消化物中的原生 NRB 会增长到高丰度。气体动力学和元组学分析表明,这些 NRB 以宏基因组组装基因组 (MAGs) 的形式恢复,是通过收获产甲烷菌的发酵中间体而生长的。财团。分离了三个 NRB,其中一个与回收的脱氯单胞菌 MAG 相匹配蛋白质组学认为它是 N 2的主要生产者富集中的O-还原酶。虽然分离株含有完全反硝化途径所需的基因,因此可以产生和隔离 N 2 O,但它们的调节特性预测它们充当土壤中的 N 2 O 汇,这已通过实验得到证实。分离物在消化物中通过有氧呼吸生长,用这些富含 NRB 的消化物施肥减少了土壤中的 N 2 O 排放。我们在土壤中使用 NRB 进行低成本和大规模接种的消化物可以作为未来应用这种强大仪器来改造土壤微生物组的蓝图,无论是用于促进植物生长、生物修复或任何其他理想功能.

更新日期:2021-09-06
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