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MicroRNA Dysregulation in the Hippocampus of Rats with Noise-Induced Hearing Loss
Oxidative Medicine and Cellular Longevity ( IF 7.310 ) Pub Date : 2021-09-06 , DOI: 10.1155/2021/1377195
Seungmin Ha 1 , Kyung Woon Kim 1 , So Min Lee 1 , Chang Ho Lee 1 , So Young Kim 1
Affiliation  

Although hippocampal changes due to noise-induced hearing loss have been suggested, little is known about the miRNA levels due to these hippocampal changes. Three-week-old Sprague-Dawley rats were divided into noise and control groups ( per group). The noise group rats were exposed to white Gaussian noise (115 dB SPL, 4 hours per day) for three days. One day after noise exposure, the hippocampi of rats were harvested and miRNA expressions were analyzed using the Affymetrix miRNA 4.0 microarray ( per group). The predicted target genes of each miRNA were retrieved, and the pathways related to the predicted target genes were analyzed. miR-758-5p, miR-210-5p, miR-370-5p, miR-652-5p, miR-3544, miR-128-1-5p, miR-665, miR-188-5p, and miR-874-5p expression increased in the hippocampal tissue of the noise group compared to that in the control group. The overlapping predicted target genes included Bend4, Creb1, Adcy6, Creb5, Kcnj9, and Pten. The pathways related to these genes were the estrogen signaling pathway, vasopressin-regulated water reabsorption, thyroid hormone synthesis, aldosterone synthesis and secretion, insulin secretion, circadian entrainment, insulin resistance, cholinergic synapse, dopaminergic synapse, cGMP-PKG signaling pathway, cAMP signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway, and AMPK signaling pathway. miR-448-3p, miR204-5p, and miR-204-3p expression decreased in the hippocampal tissue of the noise group compared to that in the control group. The overlapping predicted target genes of these three miRNAs were Rps6kas, Nfactc3, Rictor, Spred1, Cdh4, Cdh6, Dvl3, and Rcyt1b. Pathway analysis suggested that the Wnt signaling pathway is related to Dvl3 and Nfactc3. Noise-induced hearing loss dysregulates miR-758-5p, miR210-5p, miR370-5p, miR-652-5p, miR-3544, miR-128-1-5p, miR-665, miR-188-5p, miR-874-5p, miR-448-3p, miR-204-5p, miR-204-3p, and miR-140-5p expression in the hippocampus. These miRNAs have been predicted to be associated with hormonal, inflammatory, and synaptic pathways.

中文翻译:

噪声性听力损失大鼠海马区 MicroRNA 失调

尽管已经提出由于噪声引起的听力损失引起的海马变化,但对这些海马变化引起的 miRNA 水平知之甚少。三周龄的 Sprague-Dawley 大鼠分为噪音组和对照组(每组)。噪声组大鼠暴露于高斯白噪声(115 dB SPL,每天 4 小时)三天。噪声暴露一天后,采集大鼠海马体并使用 Affymetrix miRNA 4.0 微阵列分析 miRNA 的表达。每组)。检索每个miRNA的预测靶基因,并分析与预测靶基因相关的通路。miR-758-5p、miR-210-5p、miR-370-5p、miR-652-5p、miR-3544、miR-128-1-5p、miR-665、miR-188-5p和miR-874与对照组相比,噪声组海马组织中-5p的表达增加。重叠的预测靶基因包括Bend4Creb1Adcy6Creb5Kcnj9Pten. 与这些基因相关的通路是雌激素信号通路、加压素调节的水重吸收、甲状腺激素合成、醛固酮合成和分泌、胰岛素分泌、昼夜节律、胰岛素抵抗、胆碱能突触、多巴胺能突触、cGMP-PKG信号通路、cAMP信号通路通路、PI3K-Akt 信号通路、TNF 信号通路和 AMPK 信号通路。与对照组相比,噪声组海马组织中miR-448-3p、miR204-5p和miR-204-3p的表达降低。这三个 miRNA 的重叠预测靶基因是Rps6kasNfactc3RictorSpred1Cdh4Cdh6Dvl3Rcyt1b。通路分析表明Wnt信号通路与Dvl3Nfactc3有关。噪音引起的听力损失失调 miR-758-5p、miR210-5p、miR370-5p、miR-652-5p、miR-3544、miR-128-1-5p、miR-665、miR-188-5p、miR- 874-5p、miR-448-3p、miR-204-5p、miR-204-3p 和 miR-140-5p 在海马中的表达。已预测这些 miRNA 与激素、炎症和突触通路有关。
更新日期:2021-09-06
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