Aims

To analyze the biological effects of the cements Relyx Unicem 2, Panavia V5, Multilink Hybrid Abutment and SoloCem on human gingival fibroblast cells (HGFs).

Materials and methods

HGFs were exposed to different eluates (n = 40) of the studied resin-based cements. Their cytotoxic effects and influence on cell migration were assessed using MTT and wound-healing assays, respectively. Level of HGF attachment, cell morphology and F-actin cytoskeleton content after exposition to the different eluates were analyzed by scanning electron microscopy (SEM) and confocal microscopy analysis, respectively. The levels of intracellular reactive oxygen species (ROS) produced by the eluates of the different cements were also determined by flow cytometry. Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey´s test.

Results

Eluates of SoloCem significantly reduces the viability of HGFs (69% reduction compared to control at 48 h). Cell migration of HGFs in presence of undiluted SoloCem eluates was significantly lower than in the control (88% open wound area at 24 h). Contrarily, migration speed with Multilynk eluates was similar to that of the control group at all periods of time and all dilutions studied. SEM analysis showed very few cells in SoloCem group, and a moderate cell growth in Multilink, Panavia and Relyx groups were detected. Finally, ROS levels detected in HGFs treated with the more concentrated SoloCem and Relyx dilutions were significantly enhanced compared with that in the control cells or the other groups (44% and 11% ROS positive cells, respectively).

Conclusions

The results obtained in the present work suggest that Multilink hybrid abutment has better biological properties and lower cytotoxicity for cementing implant crowns on abutments.

中文翻译：

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牙冠种植体双固化和自固化基台粘固剂对人牙龈成纤维细胞生物学特性的影响

宗旨

分析 Relyx Unicem 2、Panavia V5、Multilink Hybrid Abutment 和 SoloCem 粘固剂对人牙龈成纤维细胞 (HGF) 的生物学效应。

材料和方法

HGF 暴露于所研究的树脂基水泥的不同洗脱液 (n = 40)。它们的细胞毒性作用和对细胞迁移的影响分别使用 MTT 和伤口愈合试验进行评估。分别通过扫描电子显微镜 (SEM) 和共聚焦显微镜分析来分析暴露于不同洗脱液后的 HGF 附着水平、细胞形态和 F-肌动蛋白细胞骨架含量。由不同水泥的洗脱液产生的细胞内活性氧 (ROS) 的水平也通过流式细胞术测定。数据通过单向方差分析 (ANOVA) 和 Tukey 检验进行分析。

结果

SoloCem 的洗脱液显着降低了 HGFs 的活力（与 48 小时的对照相比降低了 69%）。在未稀释的 SoloCem 洗脱液存在下，HGF 的细胞迁移显着低于对照（24 小时开放伤口面积为 88%）。相反，Multilynk 洗脱液的迁移速度在所有时间段和研究的所有稀释液中都与对照组相似。SEM 分析显示 SoloCem 组中细胞很少，而在 Multilink、Panavia 和 Relyx 组中检测到中度细胞生长。最后，与对照细胞或其他组（分别为 44% 和 11% ROS 阳性细胞）相比，在用更浓的 SoloCem 和 Relyx 稀释液处理的 HGF 中检测到的 ROS 水平显着提高。

结论

目前工作中获得的结果表明，Multilink 混合基台具有更好的生物学特性和更低的细胞毒性，用于在基台上粘合种植体冠。