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GHRH expression plasmid improves osteoporosis and skin damage in aged mice
Growth Hormone and IGF Research ( IF 1.4 ) Pub Date : 2021-09-04 , DOI: 10.1016/j.ghir.2021.101429
Rui Ye 1 , Hai-Long Wang 1 , De-Wei Zeng 1 , Ting Chen 1 , Jia-Jie Sun 1 , Qian-Yun Xi 1 , Yong-Liang Zhang 1
Affiliation  

The hormone secretion of GHRH-GH-IGF-1 axis in animals was decreased as aging. These hormones play an important role in maintaining bone mass and bone structure, and also affect the normal structure and function of the skin. We used plasmid-based technology to deliver growth hormone releasing hormone (GHRH) to elderly mice. In the current study, 80 and 120 μg/kg pVAX-GHRH plasmid expression plasmid were injected into old mice, the serum GHRH and insulin-like growth factor-1(IGF-1) content were increased within three weeks (P < 0.05). In the groups of 80 and 120 μg/kg plasmid, the content of procollagen type I N-terminal pro-peptide (PINP) in the serum was increased(P < 0.05), and the content of C-terminal telopeptides of type I collagen (CTX-1) in the serum was reduced significantly (P < 0.05). Furthermore, the expression of osteoprotegerin (OPG) and osteocalcin (OCN) in the femur also was increased(P < 0.05). The bone mineral density(BMD)、trabecular bone volume (BV/TV) and trabecular number(Tb.N) of mouse femur were increased significantly (P < 0.05) and trabecular separation(Tb.Sp) was decreased(P < 0.05). There were more trabecular bones in the bone marrow cavity and the trabecular bones are thicker in the groups of 80 and 120 μg/kg plasmid relative to control. The superoxide dismutase (SOD) content in the skin was increased(P < 0.05), and the malondialdehyde (MDA) content was reduced significantly (P < 0.05). Meanwhile, the skin moisture content also increased significantly(P < 0.05). Moreover, the expression of matrix metalloproteinase 3(MMP3) and matrix metalloproteinase 9(MMP9) was decreased in the skin(P < 0.05). The thickness of the dermis and epidermis of the skin had increased significantly(P < 0.05). Skin structure is more dense and complete in the two groups. These results indicate that 80 and 120 μg/kg plasmid-mediated GHRH supplementation can improve osteoporosis and skin aging in aged mice.



中文翻译:

GHRH 表达质粒可改善老年小鼠的骨质疏松症和皮肤损伤

动物体内GHRH-GH-IGF-1轴的激素分泌随着年龄的增长而减少。这些激素在维持骨量和骨骼结构方面发挥着重要作用,也会影响皮肤的正常结构和功能。我们使用基于质粒的技术向老年小鼠提供生长激素释放激素 (GHRH)。本研究将 80 和 120 μg/kg pVAX-GHRH 质粒表达质粒注射到老年小鼠体内,三周内血清 GHRH 和胰岛素样生长因子-1(IGF-1)含量增加(P  < 0.05) . 80、120 μg/kg质粒组血清中I型前胶原N端前肽(PINP)含量升高(P  < 0.05),I型胶原C端端肽含量升高(P < 0.05)血清中的 (CTX-1) 显着降低 (P  < 0.05)。此外,股骨中骨保护素(OPG)和骨钙素(OCN)的表达也增加(P  < 0.05)。小鼠股骨骨密度(BMD)、骨小梁体积(BV/TV)和小梁数(Tb.N)显着增加(P  < 0.05),小梁分离度(Tb.Sp)降低(P < 0.05) . 80和120μg/kg质粒组骨髓腔内骨小梁较对照组多,骨小梁较粗。皮肤超氧化物歧化酶(SOD)含量升高(P  < 0.05),丙二醛(MDA)含量显着降低(P  < 0.05)。同时,皮肤含水量也显着增加(P < 0.05)。此外,皮肤基质金属蛋白酶3(MMP3)和基质金属蛋白酶9(MMP9)的表达降低(P  < 0.05)。皮肤真皮和表皮厚度明显增加(P  < 0.05)。两组的皮肤结构更加致密和完整。这些结果表明,80 和 120 μg/kg 质粒介导的 GHRH 补充剂可以改善老年小鼠的骨质疏松症和皮肤老化。

更新日期:2021-09-08
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