Objective

To determine whether the effect of dentin moisture on the etch-and-rinse bonding may be minimized by dry-bonding protocols utilizing aqueous or ethanolic dimethyl sulfoxide (DMSO) pretreatments.

Methods

H₃PO₄-etched mid-coronal dentin surfaces from human molars were randomly blot- or air-dried for 30 s and pretreated with DMSO/H₂O or DMSO/EtOH solutions. Untreated samples served as control. Moisture control was performed by either blot- or air-drying. Samples were bonded with a multistep etch-and-rinse adhesive. Restored crown segments (n = 8/group) were stored in distilled water for 24 h and sectioned for microtensile bond strength testing. Resin-dentin beams (0.8 mm²) were tested under tension until fracture (0.5 mm/min) after 24 h and two years of storage in artificial saliva at 37 °C.

SEM nanoleakage evaluation was performed on aged samples. Collagen wettability was also measured by sessile drops of the hydrophilic and hydrophobic bonding resins (n = 8/group). Data were examined by factorial ANOVA followed by the Tukey test (α = 0.05).

Results

Dry bonding to untreated collagen produced inferior immediate and long-term bond strengths than wet bonding (p < 0.05). Regardless of initial hydration and moisture control, DMSO-dry bonding produced initially higher and stable bond strengths after aging (p < 0.05). DMSO-pretreated groups presented improved collagen wettability with lower silver uptake (p < 0.05).

Significance

Despite the common belief that etch-and-rinse adhesives must be applied onto moist collagen, DMSO-dry bonding protocols not only improved bonding performance and hybrid layer integrity, but also brought more versatility to collagen hybridization by reducing overdrying-related issues.

中文翻译：

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与牙本质干粘合：拓宽湿气谱并增加蚀刻和冲洗粘合剂的润湿性

客观的

确定是否可以通过使用水性或乙醇二甲基亚砜 (DMSO) 预处理的干粘合协议来最大限度地减少牙本质水分对蚀刻和冲洗粘合的影响。

方法

来自人类磨牙的H ₃ PO ₄蚀刻的中冠牙本质表面随机吸干或风干 30 秒，并用 DMSO/H ₂ O 或 DMSO/EtOH 溶液预处理。未处理的样品用作对照。通过印迹干燥或风干进行水分控制。样品用多步蚀刻和冲洗粘合剂粘合。修复的牙冠节段（n = 8/组）在蒸馏水中储存 24 小时，然后切片进行微拉伸粘合强度测试。树脂-牙本质梁 (0.8 mm ² ) 在张力下进行测试，直至在 37 °C 下在人工唾液中储存 24 小时和两年后断裂 (0.5 mm/min)。

对老化的样品进行 SEM 纳米泄漏评估。胶原润湿性也通过亲水性和疏水性粘合树脂（n = 8/组）的固着滴来测量。数据通过因子方差分析和 Tukey 检验 (α = 0.05) 进行检查。

结果

与未处理的胶原蛋白干粘合产生的即时和长期粘合强度低于湿粘合 ( p < 0.05)。不管最初的水化和水分控制如何，DMSO 干粘合在老化后最初产生更高且稳定的粘合强度 ( p < 0.05)。DMSO 预处理组表现出改善的胶原润湿性和较低的银吸收 ( p < 0.05)。

意义

尽管人们普遍认为必须将蚀刻和冲洗粘合剂涂在潮湿的胶原蛋白上，但 DMSO 干粘合协议不仅提高了粘合性能和混合层的完整性，而且通过减少与过度干燥相关的问题，为胶原蛋白杂交带来了更多的多功能性。