STimulated Emission Depletion (STED) microscopy attains super-resolution in biological imaging beyond the diffraction limit. Here, we give a concise protocol to construct a dual-pulse STED setup with one super-continuum laser. Moreover, a flexible and dismountable Bessel modulation module is introduced for potential 2D-stack STED imaging. Experiments and notices are introduced in detail, with discussion on some important check-points for STED, such as detector saturation. Finally, the results validate the system working.

中文翻译：

---

具有贝塞尔调制的单源双脉冲受激发射耗尽设置协议

受激发射损耗 (STED) 显微镜在超出衍射极限的生物成像中获得超分辨率。在这里，我们给出了一个简洁的协议，用一个超连续激光器构建双脉冲 STED 设置。此外，为潜在的二维堆叠 STED 成像引入了灵活且可拆卸的贝塞尔调制模块。详细介绍了实验和注意事项，并讨论了STED的一些重要检查点，例如探测器饱和度。最后，结果验证了系统的工作。