Autofluorescence of blood has been explored as a label free approach for detection of cell types, as well as for diagnosis and detection of infection, cancer, and other diseases. Although blood autofluorescence is used to indicate the presence of several physiological abnormalities with high sensitivity, it often lacks disease specificity due to use of a limited number of fluorophores in the detection of several abnormal conditions. In addition, the measurement of autofluorescence is sensitive to the type of sample, sample preparation, and spectroscopy method used for the measurement. Therefore, while current blood autofluorescence detection approaches may not be suitable for primary clinical diagnosis, it certainly has tremendous potential in developing methods for large scale screening that can identify high risk groups for further diagnosis using highly specific diagnostic tests. This review discusses the source of blood autofluorescence, the role of spectroscopy methods, and various applications that have used autofluorescence of blood, to explore the potential of blood autofluorescence in biomedical research and clinical applications.

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血液自发荧光及其在生物医学和临床研究中的应用

血液的自发荧光已被探索为检测细胞类型以及诊断和检测感染、癌症和其他疾病的无标记方法。尽管血液自发荧光用于以高灵敏度指示存在几种生理异常，但由于在检测几种异常情况时使用有限数量的荧光团，它通常缺乏疾病特异性。此外，自发荧光的测量对样品类型、样品制备和用于测量的光谱方法很敏感。因此，虽然目前的血液自发荧光检测方法可能不适合初级临床诊断，它在开发大规模筛查方法方面无疑具有巨大潜力，该方法可以使用高度特异性的诊断测试识别高风险人群以进行进一步诊断。这篇综述讨论了血液自发荧光的来源、光谱学方法的作用以及使用血液自发荧光的各种应用，以探索血液自发荧光在生物医学研究和临床应用中的潜力。