Dermal fibroblasts (DFs) share several qualities with mesenchymal stem cell/multipotent stromal cells (MSCs) derived from various tissues, including adipose-derived stromal/stem cells (ASCs). ASCs and DFs are morphologically comparable and both cell types can be culture expanded through the utilization of their plastic-adherence properties. Despite these similar characteristics, numerous studies indicate that ASC and DF display distinct therapeutic benefits in clinical applications. To more accurately distinguish between these cell types, human DFs and ASCs isolated from three individual donors were analyzed for multipotency and cell surface marker expressions. The detection of cell surface markers, CD29, CD34, CD44, CD73, CD90, and CD105, were used for phenotypic characterization of the DFs and ASCs. Furthermore, both cell types underwent lineage differentiation based on histochemical staining and the expression of adipogenic related genes, CCAAT/Enhancer-Binding Protein alpha (CEBPα), Peroxisome proliferator-activated receptor gamma (PPARγ), UCP1, Leptin (LEP), and Adiponectin (ADIPOQ); and osteogenic related genes, Runt related transcription factor 2 (Runx2), Alkaline phosphatase (ALPL), Osteocalcin (OCN), and Osteopontin (OPN). Evidence provided by this study demonstrates similarities between donor-matched ASC and DF with respect to morphology, surface marker expression, differentiation potential, and gene expression, although appearance of enhanced adipogenesis in the ASC based solely on spectrophotometric analyses with no significant difference in real-time polymerase chain reaction detection of adipogenic biomarkers. Thus, there is substantial overlap between the ASC and DF phenotypes based on biochemical and differentiation metrics.

中文翻译：

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人脂肪来源的基质/干细胞和真皮成纤维细胞的比较分析

真皮成纤维细胞 (DFs) 与源自各种组织的间充质干细胞/多能基质细胞 (MSCs) 具有多种特性，包括脂肪来源的基质/干细胞 (ASCs)。ASC 和 DF 在形态上具有可比性，两种细胞类型都可以通过利用它们的塑料粘附特性进行培养。尽管有这些相似的特征，但大量研究表明 ASC 和 DF 在临床应用中显示出不同的治疗益处。为了更准确地区分这些细胞类型，分析了从三个个体供体分离的人类 DF 和 ASC 的多能性和细胞表面标记表达。细胞表面标志物 CD29、CD34、CD44、CD73、CD90 和 CD105 的检测用于 DF 和 ASC 的表型表征。此外，根据组织化学染色和脂肪形成相关基因 CCAAT/增强子结合蛋白 α (CEBPα)、过氧化物酶体增殖物激活受体 γ (PPARγ)、UCP1、瘦素 (LEP) 和脂联素 (ADIPOQ) 的表达，两种细胞类型都经历了谱系分化); 和成骨相关基因、Runt 相关转录因子 2 (Runx2)、碱性磷酸酶 (ALPL)、骨钙素 (OCN) 和骨桥蛋白 (OPN)。本研究提供的证据表明，供体匹配的 ASC 和 DF 在形态、表面标志物表达、分化潜能和基因表达方面具有相似性，尽管仅基于分光光度分析的 ASC 中脂肪生成增强的出现，在实际中没有显着差异。脂肪形成生物标志物的时间聚合酶链反应检测。因此，