High-dimensional cytometry experiments measuring 20–50 cellular markers have become routine in many laboratories. The increased complexity of these datasets requires added rigor during the experimental planning and the subsequent manual and computational data analysis to avoid artefacts and misinterpretation of results. Here we discuss pitfalls frequently encountered during high-dimensional cytometry data analysis and aim to provide a basic framework and recommendations for reporting and analyzing these datasets.

中文翻译：

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困惑者的更新指南：高维时代的细胞术

测量 20-50 个细胞标记的高维细胞计数实验已成为许多实验室的常规操作。这些数据集的复杂性增加需要在实验计划和随后的手动和计算数据分析过程中更加严格，以避免伪影和对结果的误解。在这里，我们讨论了在高维细胞仪数据分析过程中经常遇到的陷阱，旨在为报告和分析这些数据集提供一个基本框架和建议。