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Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs
Nature Cell Biology ( IF 21.3 ) Pub Date : 2021-09-06 , DOI: 10.1038/s41556-021-00746-2
Zuzana Loubalova 1 , Helena Fulka 1, 2 , Filip Horvat 1, 3 , Josef Pasulka 1 , Radek Malik 1 , Michiko Hirose 4 , Atsuo Ogura 4, 5 , Petr Svoboda 1
Affiliation  

PIWI-interacting RNAs (piRNAs) support the germline by suppressing retrotransposons. Studies of the pathway in mice have strongly shaped the view that mammalian piRNAs are essential for male but not for female fertility. Here, we report that the role of the piRNA pathway substantially differs in golden hamsters (Mesocricetus auratus), the piRNA pathway setup of which more closely resembles that of other mammals, including humans. The loss of the Mov10l1 RNA helicase—an essential piRNA biogenesis factor—leads to striking phenotypes in both sexes. In contrast to mice, female Mov10l1–/– hamsters are sterile because their oocytes do not sustain zygotic development. Furthermore, Mov10l1–/– male hamsters have impaired establishment of spermatogonia accompanied by transcriptome dysregulation and an expression surge of a young retrotransposon subfamily. Our results show that the mammalian piRNA pathway has essential roles in both sexes and its adaptive nature allows it to manage emerging genomic threats and acquire new critical roles in the germline.



中文翻译:

黄金仓鼠精原细胞和可育卵母细胞的形成需要 piRNA

PIWI 相互作用的 RNA (piRNA) 通过抑制反转录转座子来支持生殖系。对小鼠途径的研究强烈地形成了这样一种观点,即哺乳动物的 piRNA 对雄性至关重要,但对雌性生育能力却不是。在这里,我们报告了 piRNA 通路在金仓鼠 ( Mesocricetus auratus )中的作用大不相同,其 piRNA 通路设置更类似于其他哺乳动物,包括人类。Mov10l1 RNA 解旋酶(一种重要的 piRNA 生物发生因子)的缺失导致两性中出现惊人的表型。与小鼠相比,雌性Mov10l1 –/–仓鼠是不育的,因为它们的卵母细胞不能维持合子发育。此外,Mov10l1 –/–雄性仓鼠的精原细胞建立受损,伴有转录组失调和年轻的反转录转座子亚家族的表达激增。我们的研究结果表明,哺乳动物 piRNA 通路在两性中都具有重要作用,其适应性使其能够管理新出现的基因组威胁并在生殖系中获得新的关键作用。

更新日期:2021-09-06
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