Despite the widespread application of point-of-care lateral flow tests, the viscosity dependence of these assay results remains a significant challenge. Here, we employ centrifugal microfluidic flow control through the nitrocellulose membrane of the strip to eliminate the viscosity bias. The key feature is the balancing of the sample flow into the cassette of the lateral flow test with the air flow out of the cassette. A viscosity-independent flow rate of 3.01 ± 0.18 µl/min (±6%) is demonstrated for samples with viscosities ranging from 1.1 mPas to 24 mPas, a factor greater than 20. In a model human IgG lateral flow assay, signal-intensity shifts caused by varying the sample viscosity from 1.1 mPas to 2.3 mPas could be reduced by more than 84%.

尽管即时侧流测试得到广泛应用，但这些测定结果的粘度依赖性仍然是一个重大挑战。在这里，我们通过条带的硝化纤维素膜采用离心微流体流量控制来消除粘度偏差。关键特征是流入侧流测试盒的样品流与流出盒的气流之间的平衡。对于粘度范围为 1.1 mPas 至 24 mPas（大于 20 的系数）的样品，其与粘度无关的流速为 3.01 ± 0.18 µl/min (±6%)。由于将样品粘度从 1.1 mPas 改变为 2.3 mPas 而引起的变化可以减少 84% 以上。