Syntaxin4 (STX4) gene encodes the protein STX4, a member of soluble N-ethylmaleimide-sensitive factor attachment protein receptors protein, playing a vital role in cell invadopodium formation and invasion, which is associated with the malignant progression of various human cancers. However, the expression and prognostic significance of STX4 in kidney renal clear cell carcinoma (KIRC) remain to be investigated. In this study, we collected the mRNA expression of STX4 in 535 KIRC patients from The Cancer Genome Atlasthrough the University of California Santa Cruz Xena database platform. Then we explored the expression of STX4 in KIRC, and the relationship with clinicopathological characteristics and prognostic value. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes function enrichment analyses were used to explore the potential mechanism of STX4 in KIRC. qRT-PCR analysis was performed toverify the above results with real world tissue specimens. The results indicated that STX4 was up-expressed in KIRC, and were associated with higher histological grade, advanced stage, and poorer prognosis. Moreover, elevated STX4 expression is an independent risk factor for KIRC. qRT-PCR analysis showed that STX4 was significantly elevated in 10 paired of KIRC samples compared to normal samples. Functional enrichment analysis indicated that endo/exocytosis, autophagy, mTOR signaling pathway, and NOD-like receptor signaling pathway were enriched. In summary, STX4 is constantly up-expressed in KIRC tissues, associated with a poor prognosis. We suggest that it can be an effective biomarker for the prognosis of KIRC and may be a novel therapeutic target in KIRC.

中文翻译：

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Syntaxin4在肾肾透明细胞癌中的上调表达及预后意义

Syntaxin4（STX4）基因编码蛋白STX4，是可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体蛋白的成员，在细胞侵袭伪足形成和侵袭中起重要作用，与多种人类癌症的恶性进展有关。然而，STX4在肾肾透明细胞癌（KIRC）中的表达和预后意义仍有待研究。在这项研究中，我们通过加利福尼亚大学圣克鲁斯 Xena 数据库平台从癌症基因组图谱中收集了 535 名 KIRC 患者中 STX4 的 mRNA 表达。然后我们探讨了STX4在KIRC中的表达，以及与临床病理特征和预后价值的关系。Gene Ontology和Kyoto Encyclopedia of Genes and Genomes功能富集分析被用来探索STX4在KIRC中的潜在机制。进行 qRT-PCR 分析以使用真实世界的组织标本验证上述结果。结果表明，STX4 在 KIRC 中上调，并与较高的组织学分级、晚期和较差的预后相关。此外，STX4 表达升高是 KIRC 的独立危险因素。qRT-PCR 分析表明，与正常样品相比，10 对 KIRC 样品中的 STX4 显着升高。功能富集分析表明胞内/胞吐作用、自噬、mTOR信号通路和NOD样受体信号通路被富集。总之，STX4 在 KIRC 组织中不断上表达，与预后不良有关。