Diseases bring about the need for interventions that pinpoint each specific aspect of the illness. Commonly, remission of a complex disease is accomplished by mixing treatments, medications, and therapeutics together in a fashion where they may negatively interact with each other or never arrive at the diseased site as a systemic heterogeneous mixture. Chronic wounds display intricacy as they are very localized and have their own environment where tissue deconstruction due to high levels of numerous proteases outweighs normal tissue reconstruction. This idea leads to the necessity of a protein that contains low diffusivity rates for localized treatment, strength against high concentrations of proteolytic species that lead to degradation of short chain peptides, while encompassing broad inhibitory effects against multiple proteases. Elastin-like peptides are an attractive, thermoresponsive, protein-based drug delivery partner as they contain low diffusivity and serve as a stable architecture for short chain peptide fusion. In this project, a novel elastin-like peptide-based protein has been created to target the inhibition of both human neutrophil elastase and matrix metalloprotease-2. As a biologic, this is unique as it is a protein with specific biological activities against multiple proteases, ultimately displaying the potential to mix and match differing biologically active peptides within one amino acid sequence.

中文翻译：

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混合融合蛋白作为双重蛋白酶抑制剂用于慢性伤口的愈合

疾病导致需要针对疾病的每个特定方面进行干预。通常，复杂疾病的缓解是通过将治疗、药物和治疗剂以一种方式混合在一起来实现的，在这种方式中，它们可能会相互产生负面影响，或者永远不会以全身异质混合物的形式到达患病部位。慢性伤口显示出复杂性，因为它们非常局部并且有自己的环境，其中由于大量蛋白酶而导致的组织解构超过了正常组织的重建。这个想法导致了一种蛋白质的必要性，该蛋白质包含用于局部治疗的低扩散率，对导致短链肽降解的高浓度蛋白水解物质具有强度，同时对多种蛋白酶具有广泛的抑制作用。弹性蛋白样肽是一种有吸引力的、热响应性的、基于蛋白质的药物递送伙伴，因为它们具有低扩散性并作为短链肽融合的稳定结构。在这个项目中，已经创建了一种新的弹性蛋白样肽基蛋白质，以靶向抑制人中性粒细胞弹性蛋白酶和基质金属蛋白酶-2。作为一种生物制品，这是一种独特的蛋白质，因为它是一种具有针对多种蛋白酶的特定生物活性的蛋白质，最终显示出在一个氨基酸序列中混合和匹配不同生物活性肽的潜力。一种新的弹性蛋白样肽基蛋白质已被创造出来，用于靶向抑制人中性粒细胞弹性蛋白酶和基质金属蛋白酶-2。作为一种生物制品，这是一种独特的蛋白质，因为它是一种具有针对多种蛋白酶的特定生物活性的蛋白质，最终显示出在一个氨基酸序列中混合和匹配不同生物活性肽的潜力。一种新的弹性蛋白样肽基蛋白质已被创造出来，用于靶向抑制人中性粒细胞弹性蛋白酶和基质金属蛋白酶-2。作为一种生物制品，这是一种独特的蛋白质，因为它是一种具有针对多种蛋白酶的特定生物活性的蛋白质，最终显示出在一个氨基酸序列中混合和匹配不同生物活性肽的潜力。