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Isolation and identification of the oral bacteria and their characterization for bacteriocin production in the oral cavity
Saudi Journal of Biological Sciences ( IF 4.4 ) Pub Date : 2021-09-06 , DOI: 10.1016/j.sjbs.2021.08.096
Saad Alghamdi 1
Affiliation  

Oral cavity is a diverse ecosystem which harbors immense diversity of microorganisms like fungi, virus and bacteria. Some of these microorganisms are involved in causing multiple infections. Oral flora is continuously changing due to connection with the external environment and produce bacteriocin against each other to compete for nutrient in this mini ecosystem. Current study was aimed to explore and compare the bacterial fauna of both healthy and non-healthy dental samples, by isolation and identification with biochemical tests to characterize the bacteriocin production. During study 120 swabs were taken from both healthy and unhealthy subjects. Samples were collected from the dental clinics of Makkah City, in sterile eppendorfs containing 1 ml nutrient broth, and were incubated overnight using shaking incubator. Bacteria were isolated following identification through Gram staining, microscopy and biochemical test. Total 15 strains of bacteria were isolated during the study amongst which 8 strains were gram positive and 7 strains were gram negative. The most dominant species of the gram positive strains was Streptococcus pneumoniae (n = 26). On the other hand, Escherichia coli (n = 26) was the prominent specie amongst the gram negative strains. Overall, the dominated family was Enterobacteriaceae (19.36%) followed by Streptococcaceae with 13.83% abundance. One of the most cariogenic strain Klebsiella pneumoniae (n = 14) was also isolated. The bacterial strain diversity between these two type of ecosystem was approximately the same, with slight variation in Shannon (HS:2.627187, NHS:2.653594) and Simpson diversity (HS:0.923461, NHS: 0.92684) index. The current research revealed that bacteriocin production in the Enterobacter species was prominent against Escherichia coli and Klebsiella pneumoniae. Apart from this other strains like Klebsiella pneumoniae and Exiguobacterium spp were also able to produce bacteriocin against Enterobacter species and Bacillus cereus respectively.



中文翻译:

口腔细菌的分离和鉴定及其在口腔中产生细菌素的表征

口腔是一个多样化的生态系统,其中蕴藏着极其多样的微生物,如真菌、病毒和细菌。其中一些微生物与引起多重感染有关。口腔菌群因与外界环境的联系而不断发生变化,相互产生细菌素,在这个小生态系统中争夺养分。目前的研究旨在探索和比较健康和非健康牙齿样本的细菌群落,通过生化测试的分离和鉴定来表征细菌素的产生。在研究期间,从健康和不健康的受试者身上采集了 120 个拭子。样品从麦加市的牙科诊所收集在含有 1 ml 营养肉汤的无菌 eppendorfs 中,并使用摇床培养过夜。通过革兰氏染色、显微镜检查和生化试验鉴定后分离出细菌。研究期间共分离出15株细菌,其中革兰氏阳性8株,革兰氏阴性7株。革兰氏阳性菌株的优势种是肺炎链球菌(n = 26)。另一方面,大肠杆菌(n = 26)是革兰氏阴性菌株中的主要物种。总体而言,占主导地位的科是肠杆菌科(19.36%),其次是链球菌科,丰度为 13.83%。还分离出一种最致龋的肺炎克雷伯菌(n = 14)。这两种生态系统之间的菌株多样性大致相同,香农(HS:2.627187,NHS:2.653594)和辛普森多样性(HS:0.923461,NHS:0.92684)指数略有变化。目前的研究表明,肠杆菌属中细菌素的产生对大肠杆菌肺炎克雷伯菌具有显着性。. 除此之外,其他菌株如肺炎克雷伯菌微小杆菌也能够分别产生针对肠杆菌属蜡状芽孢杆菌的细菌素。

更新日期:2021-09-06
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