We recently reported that silencing of the polyadenylation factor EhCFIm25 in Entamoeba histolytica, the protozoan which causes human amoebiasis, affects trophozoite proliferation, death, and virulence, suggesting that EhCFIm25 may have potential as a new biochemical target. Here, we performed a shotgun proteomic analysis to identify modulated proteins that could explain this phenotype. Data are available via ProteomeXchange with identifier PXD027784. Our results revealed changes in the abundance of 75 proteins. Interestingly, STRING analysis, functional GO-term annotations, KEGG analyses, and literature review showed that modulated proteins are mainly related to glycolysis and carbon metabolism, cytoskeleton dynamics, and parasite virulence, as well as gene expression and protein modifications. Further studies are needed to confirm the hypotheses emerging from this proteomic analysis, to thereby acquire a comprehensive view of the molecular mechanisms involved.

中文翻译：

---

通过蛋白质组学分析揭示多腺苷酸化因子 EhCFIm25 在溶组织内阿米巴中的相关性

我们最近报道了溶组织内阿米巴中多聚腺苷酸化因子 EhCFIm25 的沉默，导致人类阿米巴病的原生动物，会影响滋养体的增殖、死亡和毒力，这表明 EhCFIm25 可能具有作为新的生化靶点的潜力。在这里，我们进行了鸟枪蛋白质组学分析，以确定可以解释这种表型的调节蛋白。数据可通过 ProteomeXchange 获得，标识符为 PXD027784。我们的结果揭示了 75 种蛋白质丰度的变化。有趣的是，STRING 分析、功能性 GO 术语注释、KEGG 分析和文献综述表明，受调节的蛋白质主要与糖酵解和碳代谢、细胞骨架动力学和寄生虫毒力以及基因表达和蛋白质修饰有关。需要进一步的研究来证实这种蛋白质组学分析中出现的假设，