Allogeneic hematopoietic stem cell transplantation (HSCT) remains the key for the treatment of malignant hematological diseases, and acute graft-versus-host disease (aGVHD) that might occur after allogenic transplantation can be life threatening and promote disease recurrence. GVHD damages the various parts of the body by upregulating T helper 1 cytokines (Th1) cytokines and stimulating CD4、CD8 + T cells. GVHD can exhibit significant immunoregulatory effects, but could be easily affected by the mesenchymal stem cells (MSC) environment, and hence the MSC immunosuppressive effects on GVHD remain unpredictable. Hence, to better understand the role of MSC in the prevention and treatment of GVHD, umbilical cord derived mesenchymal stem cells (UC-MSC) were pre-treated with Chinese medicine Asarinin and IFN-γ. In the mix lymphocyte reaction, we found that Asarinin pre-treated UC-MSC can exert significantly greater inhibition towards the proliferation of CD4 and CD8 + T cells, down-regulate Th1 type cytokines, up-regulate Th2 type cytokines, and reduce the inflammatory damage to liver, lung and intestine of aGVHD mice model. Moreover, Asarinin can cooperate with IFN-γto promote UC-MSC to secrete indoleamine 2,3-dioxygenase (IDO). Our findings establish that Asarinin pre-treated UC-MSC can significantly promote the immunosuppressive effects of MSC on aGVHD after hematopoietic stem cell transplantation.

异基因造血干细胞移植（HSCT）仍然是治疗恶性血液病的关键，异基因移植后可能发生的急性移植物抗宿主病（aGVHD）可能危及生命并促进疾病复发。GVHD通过上调T辅助1细胞因子（Th1）细胞因子和刺激CD4、CD8 + T细胞来损害身体的各个部位。GVHD可以表现出显着的免疫调节作用，但很容易受到间充质干细胞（MSC）环境的影响，因此MSC对GVHD的免疫抑制作用仍然不可预测。因此，为了更好地了解MSC在预防和治疗GVHD中的作用，脐带间充质干细胞（UC-MSC）用中药细辛和IFN-γ进行预处理。在混合淋巴细胞反应中，我们发现，细辛预处理的 UC-MSC 对 CD4 和 CD8 + T 细胞的增殖有显着的抑制作用，下调 Th1 型细胞因子，上调 Th2 型细胞因子，并减少对肝、肺和aGVHD 小鼠模型的肠道。此外，细辛可以与 IFN-γ 协同促进 UC-MSC 分泌吲哚胺 2,3-双加氧酶 (IDO)。我们的研究结果表明，细辛素预处理的 UC-MSC 可以显着促进 MSC 在造血干细胞移植后对 aGVHD 的免疫抑制作用。细辛可以与 IFN-γ 协同促进 UC-MSC 分泌吲哚胺 2,3-双加氧酶 (IDO)。我们的研究结果表明，细辛素预处理的 UC-MSC 可以显着促进 MSC 在造血干细胞移植后对 aGVHD 的免疫抑制作用。细辛可以与 IFN-γ 协同促进 UC-MSC 分泌吲哚胺 2,3-双加氧酶 (IDO)。我们的研究结果表明，细辛素预处理的 UC-MSC 可以显着促进 MSC 在造血干细胞移植后对 aGVHD 的免疫抑制作用。