Heat stress is a major detriment to crop growth and yield under the current patterns of climate change. Two independent experiments of camelina and canola were conducted to assess the role of thiourea (TU) priming in modulating heat stress tolerance in camelina and canola. The experiment comprised of three factors: (i) seed priming; NP (no priming), HP (hydro-priming), TU₅₀₀ (thiourea priming at 500 mg L⁻¹), TU₁₀₀₀ (thiourea priming at 1000 mg L⁻¹), and TU₁₅₀₀ (thiourea priming at 1500 mg L⁻¹); (ii) heat stress; control (22/18 °C) and heat stress (35/25 °C) applied at 20% flowering (62 DAS); and (iii) cultivars; camelina (611 and 618) and canola (Hiola-401 and 45S42). The results indicated that the plants exposed to heat stress exhibited a significant decrease in the growth and yield of camelina and canola due to impaired physiological attributes, while activities/levels of superoxide dismutase, total soluble sugars, glycine betaine, and proline were triggered under heat stress compared with control. Heat stress decreased the photosynthetic rate and water potential by 47% and 23% in camelina and 49% and 24% in canola that led to reduction in seed yield of camelina and canola by 53% and 57%, respectively. Seed priming with thiourea improved the growth and yield attributes of both crops by maintaining the gas-exchange attributes, water relations, and upregulating the activities/levels of antioxidants compared with NP; the maximum improvement was observed with thiourea priming at TU₁₀₀₀. Cultivars 618 and 45S42 showed better antioxidant activity to maintain redox potential in camelina and canola, respectively. In crux, these findings indicated that thiourea seed priming improved the heat stress tolerance in camelina and canola, which might be attributed to higher accumulation of secondary metabolites, better antioxidative defense, and maintenance of plant water status in thiourea-primed seedlings.

在当前的气候变化模式下，热应激是对作物生长和产量的主要危害。对亚麻荠和油菜籽进行了两个独立的实验，以评估硫脲 (TU) 引发在调节亚麻荠和油菜籽热应激耐受性中的作用。该实验包括三个因素：(i) 种子引发；NP（无引发）、HP（氢引发）、TU ₅₀₀（以 500 mg L ^-1引发硫脲）、TU ₁₀₀₀（以 1000 mg L ^-1引发硫脲）和 TU ₁₅₀₀（以 1500 mg L ^-引发硫脲）¹); (ii) 热应激；控制 (22/18 °C) 和热应激 (35/25 °C) 在 20% 开花 (62 DAS) 下应用；(iii) 栽培品种；亚麻荠（611 和 618）和油菜（Hiola-401 和 45S42）。结果表明，暴露于热胁迫的植物由于生理特性受损，其生长和产量显着下降，而超氧化物歧化酶、总可溶性糖、甘氨酸甜菜碱和脯氨酸的活性/水平在热条件下被触发。压力与对照相比。热应激使亚麻荠的光合速率和水势降低了 47% 和 23%，双低油菜籽的光合速率和水势分别降低了 49% 和 24%，导致亚麻荠和双低油菜籽的种子产量分别减少了 53% 和 57%。用硫脲引发种子通过保持气体交换特性改善了两种作物的生长和产量特性，水关系，与 NP 相比，上调抗氧化剂的活性/水平；在 TU 处使用硫脲引发最大程度的改善₁₀₀₀。品种 618 和 45S42 分别显示出更好的抗氧化活性，以保持茶和油菜中的氧化还原电位。关键是，这些发现表明硫脲种子引发提高了亚麻荠和油菜的热应激耐受性，这可能归因于次生代谢物的更高积累、更好的抗氧化防御以及硫脲引发幼苗中植物水分状态的维持。