Salmonella detection and isolation rely on different selective enrichment media, which can influence which serovars are detected. The objective of this study was to compare Salmonella recovery from broiler carcass rinses using three different selective enrichment protocols and three differential plating agars. Eight prechill broiler carcasses were collected at a commercial slaughter facility. Each carcass was subjected to whole carcass rinse procedure in buffered peptone water (BPW). An aliquot of the rinse and whole carcasses in the remaining rinse were incubated as a pre-enrichment before subculturing in selective enrichment broths (Rappaport Vassiliadis [RV], Tetrathionate Hajna [TT], and TT to RV in series [TT-to-RV]). Enriched samples were streaked on the three differential agars (Hektoen Enteric [HE], Brilliant Green Sulfa [BGS], and Xylose-Lysine-Tergitol-4 [XLT-4]). Salmonella was isolated from all eight carcasses. Considering all sample preparations as independent subsamples, Salmonella was detected in 88% (128/144) of subsamples with a 100% recovery from the TT-to-RV enrichment, and 92 and 71% from RV and TT broths, individually. A high concordance in recovery on BGS versus XLT-4 agar plates was observed compared to HE versus BGS and HE versus XLT-4 plates. These data suggest that choice of pre-enrichment method, selective enrichment medium, and differential agar can influence the recovery of Salmonella from poultry samples.

中文翻译：

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从肉鸡屠体中分离沙门氏菌的选择性富集培养基和平板培养基的比较

沙门氏菌检测和分离依赖于不同的选择性富集培养基，这会影响检测到的血清型。本研究的目的是比较沙门氏菌使用三种不同的选择性富集方案和三种差异平板琼脂从肉鸡屠体冲洗液中回收。在商业屠宰场收集了八只预冷肉鸡胴体。每个屠体在缓冲蛋白胨水 (BPW) 中进行整个屠体冲洗程序。在选择性富集肉汤（Rappaport Vassiliadis [RV]、Tetrathionate Hajna [TT] 和 TT 到 RV 系列 [TT-to-RV] ]）。富集的样品在三种差异琼脂（Hektoen Enteric [HE]、Brilliant Green Sulfa [BGS] 和 Xylose-Lysine-Tergitol-4 [XLT-4]）上划线。沙门氏菌是从所有八具尸体中分离出来的。将所有样品制备视为独立的子样品，沙门氏菌在 88% (128/144) 的子样本中被检测到，从 TT 到 RV 的富集回收率为 100%，从 RV 和 TT 肉汤中分别为 92% 和 71%。与 HE 与 BGS 和 HE 与 XLT-4 琼脂平板相比，BGS 与 XLT-4 琼脂平板上的回收率高度一致。这些数据表明，预富集方法、选择性富集培养基和差异琼脂的选择会影响家禽样品中沙门氏菌的回收率。