当前位置: X-MOL 学术Clin. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding
Clinical Immunology ( IF 8.6 ) Pub Date : 2021-09-04 , DOI: 10.1016/j.clim.2021.108848
David S Pisetsky 1 , Robert Shaffer 2 , Dustin D Armstrong 2 , Diane M Spencer 3
Affiliation  

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus. Previous studies have indicated that the phosphodiester backbone is the main antigenic target, with electrostatic interactions important for high avidity. To define further these interactions, the effects of ionic strength on anti-DNA binding of SLE plasmas were assessed in association and dissociation assays by ELISA. As these studies demonstrated, increasing ionic strength to a concentration of 1000 mM NaCl reduced antibody binding although the extent of the reduction varied among samples. In dissociation assays, differences among plasmas were also observed. For one of the plasmas, binding to DNA displayed resistance to dissociation by increasing ionic strength even though these concentrations limited binding in association assays. Time course studies showed a gradual change in binding interactions. These studies indicate that anti-DNA binding can involve both electrostatic and non-electrostatic interactions, with binding in some plasmas showing evidence of hysteresis.



中文翻译:

抗 DNA 抗体与 DNA 抗原的相互作用:高亲和力结合滞后的证据

DNA 抗体(anti-DNA)是系统性红斑狼疮的血清学标志。以前的研究表明,磷酸二酯骨架是主要的抗原靶标,静电相互作用对高亲和力很重要。为了进一步定义这些相互作用,离子强度对 SLE 血浆的抗 DNA 结合的影响通过 ELISA 在结合和解离测定中进行了评估。正如这些研究表明的那样,将离子强度增加到 1000 mM NaCl 的浓度会降低抗体结合,尽管降低的程度因样品而异。在解离测定中,还观察到血浆之间的差异。对于其中一种血浆,与 DNA 的结合通过增加离子强度显示出对解离的抵抗力,即使这些浓度限制了结合测定中的结合。时间过程研究表明结合相互作用逐渐变化。这些研究表明,抗 DNA 结合可能涉及静电和非静电相互作用,在一些血浆中的结合显示出滞后现象。

更新日期:2021-09-12
down
wechat
bug