Antibacterial photodynamic therapy is a promising treatment for problematic infections caused by bacteria and fungi. Despite its undoubted effectiveness, the ultrastructural mechanism of microbial death remains not fully described and distinct organisms respond to the treatment with different efficacy. For this reason, it was decided to try imaging the process using the in situ transmission electron microscopy method. To conduct an observational experiment, the microscope was significantly modified. Liquid cell methods were used, electron doses and their influence on the sample were estimated, and a fiber-optic sample illuminator was designed and built. The modifications allowed for the light-induced characterization of photosensitizer-bacteria interaction. Microscope modification is a promising platform for further studies of light-induced phenomena in both life and material science.

抗菌光动力疗法是一种很有前途的治疗由细菌和真菌引起的问题感染。尽管其有效性毋庸置疑，但微生物死亡的超微结构机制仍未得到充分描述，不同的生物体对治疗的反应具有不同的功效。出于这个原因，决定尝试使用原位透射电子显微镜方法对该过程进行成像。为了进行观察实验，显微镜被显着修改。采用液池法，估计了电子剂量及其对样品的影响，并设计并制造了光纤样品照明器。这些修改允许光敏剂-细菌相互作用的光诱导表征。