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Thrips diversity of cotton ecosystem and the role of parthenium pollen grains in the transmission of tobacco streak virus (TSV) infection in cotton
3 Biotech ( IF 2.8 ) Pub Date : 2021-09-03 , DOI: 10.1007/s13205-021-02967-6
S Rageshwari 1, 2 , S Vinodkumar 1, 2 , P Renukadevi 3 , V G Malathi 4 , S Nakkeeran 2
Affiliation  

Survey in the cotton fields of Coimbatore and Erode districts confirmed the presence of three different thrips species including, Scirtothrips dorsalis, Scirtothrips oligochaetus, and Frankliniella occidentalis. Their identity was confirmed through morphometric analysis and molecular characterization. Tobacco streak virus (TSV) was detected in the leaves and pollen grains of both parthenium and cotton plants collected from infected cotton fields. The presence of TSV was confirmed through immuno-detection by direct antigen coating enzyme-linked immuno sorbent assay (DAC-ELISA). Further confirmation was accomplished by reverse transcriptase polymerase chain reaction (RT-PCR) using TSV coat protein-specific primers. Other than parthenium, weed plants in the cotton field such as Trianthema portulacastrum, Boerhavia diffusa, and Amaranthus sp. were also confirmed for TSV infection through RT-PCR. Parthenium hysterophorus plants acts as a silent carrier of TSV and they occasionally produced symptoms. Among all the randomly collected thrips samples, two pooled samples were detected positive for TSV through RT-PCR.



中文翻译:

棉花生态系统蓟马多样性及部分菊花粉粒在棉花烟草条纹病毒(TSV)传播中的作用

对哥印拜陀和埃罗德地区棉田的调查证实存在三种不同的蓟马物种,包括蓟马、寡毛刺蓟马和西洋富兰克林蓟马。它们的身份通过形态计量分析和分子表征得到证实。从受感染的棉田采集的parthenium 和棉花植物的叶子和花粉粒中都检测到了烟草条纹病毒(TSV)。通过直接抗原包被酶联免疫吸附试验(DAC-ELISA)进行免疫检测,证实了 TSV 的存在。使用 TSV 外壳蛋白特异性引物通过逆转录酶聚合酶链式反应 (RT-PCR) 完成进一步确认。除parthenium外,棉田中的杂草植物如马齿苋白花苋、属植物。还通过 RT-PCR 确认 TSV 感染。Parthenium hysterophorus植物作为 TSV 的沉默载体,它们偶尔会产生症状。在所有随机收集的蓟马样本中,通过 RT-PCR 检测到两个合并样本对 TSV 呈阳性。

更新日期:2021-09-04
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