There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M. bovis and M. canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milk and biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M. californicum and M. leachii were detected by using two multiplex PCRs, confirming their presence in clinical conditions in dairy cattle. The techniques used in the present study can be useful to broaden the knowledge about Mycoplasma infections in cattle, since the search for these organisms is not usually included in routine diagnoses.

关于与支原体存在相关的频率、流行病学和临床特征的信息很少。在阿根廷奶牛群中。本研究的目的是开发用于鉴定牛分枝杆菌和加拿大分枝杆菌的多重PCR 并描述支原体属 的频率。从提交给诊断实验室的临床样本中分离。在来自乳房内感染、散装牛奶和生物体液的 1548 个样本中，获得了 38 个支原体分离物。M. bovis , M. canadense , M. californicum和M. leachii 通过使用两个多重 PCR 检测到，证实了它们在奶牛的临床状况中的存在。本研究中使用的技术有助于拓宽关于牛支原体感染的知识，因为对这些微生物的搜索通常不包括在常规诊断中。