The interaction between organic molecules and biomaterial surfaces determines the fate of biomaterials during their service life, which is also the research hotspots in the field of biomaterials. To understand the mechanism of protein interaction with magnesium (Mg) degradation, alloying elements, immersion time, protein concentration and surface conditions have been previously considered for the effect of proteins on Mg degradation. However, fluid flow, as one of the critical factors, drew little attention in this case. In the present study, the effect of bovine serum albumin (BSA) and fetal bovine serum (FBS) on Mg degradation was compared under static and dynamic conditions. The results revealed that both BSA and FBS slightly decreased the degradation rate of Mg in Hanks’ balanced salt solution (HBSS) under static immersion due to the protein adsorption and the formation of a Ca/P-rich top layer on Mg surface, whereas under dynamic flow condition the degradation of Mg was significantly accelerated in the presence of BSA or FBS. The reasons seemed to stem from the weakened protein adsorption on Mg surface in this case and the dynamically enhanced interaction between proteins and ions/products in solutions, which largely weaken the combination of the top Ca/P-rich layer with the inner corrosion product layer. These results highlight the importance of testing conditions for Mg characterization in vitro and the synergistic effect between different parameters on Mg degradation.

有机分子与生物材料表面的相互作用决定了生物材料在使用寿命期间的命运，这也是生物材料领域的研究热点。为了了解蛋白质与镁 (Mg) 降解的相互作用机制，先前已经考虑了合金元素、浸泡时间、蛋白质浓度和表面条件，以了解蛋白质对镁降解的影响。然而，作为关键因素之一的流体流动在这种情况下很少引起注意。在本研究中，比较了静态和动态条件下牛血清白蛋白 (BSA) 和胎牛血清 (FBS) 对镁降解的影响。结果表明，由于蛋白质吸附和镁表面富钙/磷顶层的形成，BSA 和胎牛血清在静态浸泡下均略微降低了汉克斯平衡盐溶液 (HBSS) 中镁的降解速率，而在在动态流动条件下，Mg 的降解在 BSA 或 FBS 存在下显着加速。原因似乎是由于在这种情况下蛋白质在镁表面的吸附减弱以及溶液中蛋白质与离子/产物之间相互作用的动态增强，这在很大程度上削弱了顶部富含钙/磷的层与内部腐蚀产物层的结合. 这些结果突出了测试条件对镁表征的重要性 而在动态流动条件下，Mg 的降解在 BSA 或 FBS 的存在下显着加速。原因似乎是由于在这种情况下蛋白质在镁表面的吸附减弱以及溶液中蛋白质与离子/产物之间相互作用的动态增强，这在很大程度上削弱了顶部富含钙/磷的层与内部腐蚀产物层的结合. 这些结果突出了测试条件对镁表征的重要性 而在动态流动条件下，Mg 的降解在 BSA 或 FBS 的存在下显着加速。原因似乎是由于在这种情况下蛋白质在镁表面的吸附减弱以及溶液中蛋白质与离子/产物之间相互作用的动态增强，这在很大程度上削弱了顶部富含钙/磷的层与内部腐蚀产物层的结合. 这些结果突出了测试条件对镁表征的重要性在体外以及不同参数之间对镁降解的协同作用。