当前位置: X-MOL 学术Environ. Toxicol. Pharmacol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biochemical and developmental effects of thyroid and anti-thyroid drugs on different early life stages of Xenopus laevis
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2021-09-04 , DOI: 10.1016/j.etap.2021.103738
Filiz Boran 1 , Abbas Güngördü 1
Affiliation  

The effects of two drugs containing the synthetic thyroid hormone levothyroxine (LEV) and an anti-thyroid drug containing propylthiouracil (PTU) on the three early life stages of Xenopus laevis were evaluated with the Frog Embryo Teratogenesis Assay-Xenopus, Tadpole Toxicity Test, and Amphibian Metamorphosis Assay using biochemical and morphological markers. Tested drugs caused more effective growth retardation in stage 8 embryos than stage 46 tadpoles. Significant inhibition of biomarker enzymes has been identified in stage 46 tadpoles for both drugs. AMA test results showed that LEV-I caused progression in the developmental stage and an increase in thyroxine level in 7 days exposure and growth retardation in 21 days exposure in stage 51 tadpoles. On the other hand, increases in lactate dehydrogenase activity for both drugs in the AMA test may be due to impacted energy metabolism during sub-chronic exposure. These results also show that the sensitivity and responses of Xenopus laevis at different early developmental stages may be different when exposed to drugs.



中文翻译:

甲状腺和抗甲状腺药物对非洲爪蟾不同早期生命阶段的生化和发育影响

用青蛙胚胎致畸试验-非洲爪蟾评估了两种含有合成甲状腺激素左旋甲状腺素 (LEV) 的药物和一种含有丙硫氧嘧啶 (PTU) 的抗甲状腺药物对非洲爪蟾三个早期生命阶段的影响、蝌蚪毒性测试和使用生化和形态学标记的两栖动物变态分析。与第 46 阶段的蝌蚪相比,测试药物在第 8 阶段的胚胎中导致更有效的生长迟缓。在这两种药物的第 46 阶段蝌蚪中都发现了对生物标志物酶的显着抑制。AMA 测试结果表明,LEV-I 导致发育阶段的进展,并在暴露 7 天后导致甲状腺素水平增加,并在暴露 21 天后导致 51 阶段蝌蚪的生长迟缓。另一方面,AMA 测试中两种药物乳酸脱氢酶活性的增加可能是由于亚慢性暴露期间能量代谢受到影响。这些结果还表明非洲爪蟾的敏感性和反应 在不同的早期发育阶段接触药物时可能会有所不同。

更新日期:2021-09-04
down
wechat
bug