Lipids are the structural constituents of cell membranes and play crucial roles in plant adaptation to abiotic stresses. The aim of this study was to use glycerolipidomic and transcriptomic to analyze the changes in lipids metabolism induced by cadmium (Cd) exposure in wheat. The results indicated that Cd stress did not decrease the concentrations of monogalactosyldiacyglycerol (MGDG), phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and phosphatidic acid at 6 h, but decreased digalactosyldoacylglycerol (DGDG), MGDG, PC, phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylserine (PS) and LPC concentrations in wheat root at 24 h. Although the concentrations of highly abundant glycerolipids PC and PE were decreased, the ratios of PC/PE increased thus contributing to wheat adaptation to Cd stress. Cd did not reduce the extent of total lipid unsaturation due to the unchanged concentrations of high abundance species of C36:4, C34:2, C34:3 and C36:6 at 6 h, indicative of their roles in resisting Cd stress. The correlation analysis revealed the glycerolipids species experiencing co-metabolism under Cd stress, which is driven by the activated expression of genes related to glycerolipid metabolism, desaturation and oxylipin synthesis. This study gives insights into the changes of glycerolipids induced by Cd and the roles in wheat adaptation to Cd stress.

脂质是细胞膜的结构成分，在植物适应非生物胁迫中起着至关重要的作用。本研究的目的是使用甘油脂质组学和转录组学来分析小麦中镉 (Cd) 暴露引起的脂质代谢变化。结果表明，Cd胁迫在6 h时单半乳糖二酰基甘油（MGDG）、磷脂酰胆碱（PC）、溶血磷脂酰胆碱（LPC）和磷脂酸的浓度没有降低，但降低了双半乳糖二酰基甘油（DGDG）、MGDG、PC、磷脂酰乙醇胺（PE）、磷脂酰甘油。 （PG），24小时小麦根中的磷脂酰丝氨酸（PS）和LPC浓度。虽然高丰度甘油脂 PC 和 PE 的浓度降低了，但 PC/PE 的比例增加，从而有助于小麦对 Cd 胁迫的适应。由于 C36:4、C34:2、C34:3 和 C36:6 的高丰度物种在 6 小时时浓度不变，Cd 并未降低总脂质不饱和程度，这表明它们在抵抗 Cd 胁迫中的作用。相关性分析揭示了甘油脂物种在镉胁迫下经历共代谢，这是由与甘油脂代谢、去饱和和氧化脂合成相关的基因的激活表达驱动的。本研究揭示了镉诱导的甘油脂的变化以及小麦对镉胁迫适应的作用。这是由与甘油脂代谢、去饱和和氧化脂合成相关的基因的激活表达驱动的。本研究揭示了镉诱导的甘油脂的变化以及小麦对镉胁迫适应的作用。这是由与甘油脂代谢、去饱和和氧化脂合成相关的基因的激活表达驱动的。本研究揭示了镉诱导的甘油脂的变化以及小麦对镉胁迫适应的作用。