Purpose

To investigate the association of omentin-1 and inflammatory factors in serum and visceral adipose tissue (VAT) of women with gestational diabetes mellitus (GDM) compared to normal pregnant (NP) subjects. Furthermore, to examine their correlation with maternal clinical characteristics.

Methods

We compared 116 GDM women to 115 NP women, at the time of cesarean section. Circulating omentin-1 and pro-inflammatory (IL-1β, IL-6, TNF-α), and anti-inflammatory cytokines (IL-1RA, IL-10) were examined. Moreover, their mRNA expression in VAT, along with inflammatory factors involved in the NF-κB pathway (TLR2, TLR4, NF-κB, IKκB), were examined.

Results

Circulating omentin-1 (p = 0.022) was lower and circulating IL-1-β, IL-1RA, as well as IL-10 (p = 0.005, p = 0.007, and p = 0.015, respectively), were higher in GDM compared to NP women. Omentin-1 correlated negatively with pre-pregnancy and gestational BMI, and HOMA-IR in all women, but was not associated with cytokines. TLR2, TLR4, IL-1β, IL-1RA, IL-6, IL-10 mRNA expression in VAT was lower in GDM compared with controls (p < 0.05 all). In multivariate analysis, BMI at delivery was significantly correlated to omentin-1 concentrations in all and NP subjects. In addition, omentin-1 expression was correlated to inflammatory gene expression in all, GDM and NP, women (p < 0.05 all).

Conclusion

Serum levels and VAT gene expression of omentin-1 are not independently linked to GDM; notwithstanding, GDM women have a VAT-altered inflammatory status. In addition, no systemic association between omentin-1 and inflammatory factors was found, whereas associations between their expression in all women were observed, indicating that expression of these adipokines is linked between them regardless of GDM.

中文翻译：

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妊娠期糖尿病女性母体血浆和内脏脂肪组织中Omentin-1及其与炎症因子的关系

目的

研究与正常妊娠 (NP) 受试者相比，妊娠期糖尿病 (GDM) 女性血清和内脏脂肪组织 (VAT) 中 omentin-1 与炎症因子的关系。此外，检查它们与母体临床特征的相关性。

方法

我们在剖宫产时比较了 116 名 GDM 女性和 115 名 NP 女性。检测了循环 omentin-1 和促炎因子（IL-1β、IL-6、TNF-α）和抗炎细胞因子（IL-1RA、IL-10）。此外，还检测了它们在 VAT 中的 mRNA 表达，以及参与 NF-κB 通路（TLR2、TLR4、NF-κB、IKκB）的炎症因子。

结果

循环 omentin-1 ( p  = 0.022) 较低，循环 IL-1-β、IL-1RA 和 IL-10 (分别为p  = 0.005、p  = 0.007 和p  = 0.015) 在 GDM 中较高与NP女性相比。Omentin-1 与所有女性的孕前和妊娠 BMI 以及 HOMA-IR 呈负相关，但与细胞因子无关。与对照组相比，GDM 组 VAT 中 TLR2、TLR4、IL-1β、IL-1RA、IL-6、IL-10 mRNA 表达低于对照组（均p  < 0.05）。在多变量分析中，分娩时的 BMI 与所有受试者和 NP 受试者中的 omentin-1 浓度显着相关。此外，omentin-1 的表达与所有女性、GDM 和 NP、女性的炎症基因表达相关（均p  < 0.05）。

结论

omentin-1 的血清水平和 VAT 基因表达与 GDM 无关；尽管如此，GDM 女性具有增值税改变的炎症状态。此外，未发现 omentin-1 与炎症因子之间存在系统性关联，而在所有女性中均观察到它们的表达之间存在关联，表明这些脂肪因子的表达与 GDM 无关。