We present genomic, phylogenomic, and phenotypic taxonomic data to demonstrate that three human ear isolates represent a novel species within the genus Gulosibacter. These isolates could not be identified reliably using MALDI-TOF mass spectrometry during routine diagnostic work, but partial 16S rRNA gene sequence analysis revealed that they belonged to the genus Gulosibacter. Overall genomic relatedness indices between the draft genome sequences of the three isolates and of the type strains of established Gulosibacter species confirmed that the three isolates represented a single novel Gulosibacter species. A biochemical characterisation yielded differential tests between the novel and established Gulosibacter species, which could also be differentiated using MALDI-TOF mass spectrometry. We propose to formally classify these three isolates into Gulosibacter hominis sp. nov., with 401352-2018 ^T (= LMG 31778 ^T, CCUG 74795 ^T) as the type strain. The whole-genome sequence of strain 401352-2018 ^T has a size of 2,340,181 bp and a G+C content of 62.04 mol%. A Gulosibacter pangenome analysis revealed 467 gene clusters that were exclusively present in G. hominis genomes. While these G. hominis specific gene clusters were enriched in several COG functional categories, this analysis did not reveal functions that suggested a role in the human microbiome, nor did it explain the occurrence of G. hominis in ear infections. The absence of acquired antimicrobial resistance determinants and virulence factors in the G. hominis genomes, and an analysis of publicly available 16S rRNA gene sequences and 16S rRNA amplicon sequencing data sets suggested that G. hominis is a member of the human skin microbiota that may occasionally be involved in opportunistic infections.

我们提供基因组、系统基因组和表型分类学数据，以证明三个人耳分离株代表Gulosibacter属内的一个新物种。在常规诊断工作中，使用 MALDI-TOF 质谱法无法可靠地鉴定这些分离株，但部分 16S rRNA 基因序列分析显示它们属于Gulosibacter属。三个分离株的基因组序列草图和已建立的古罗杆菌属物种的典型菌株之间的总体基因组相关性指数证实，这三个分离株代表了一个新的古罗杆菌属物种。生化表征产生了新的和已建立的之间的差异测试Gulosibacter物种，也可以使用 MALDI-TOF 质谱法进行区分。我们建议将这三个分离株正式分类为Gulosibacter hominis sp。11 月.,以 401352-2018  ^T (= LMG 31778  ^T , CCUG 74795  ^T ) 作为类型菌株。菌株401352-2018  ^T的全基因组序列大小为2,340,181 bp，G+C含量为62.04 mol%。甲Gulosibacter泛基因组分析显示，分别只存在于467个基因簇G.人型支原体基因组。虽然这些G. hominis特定的基因簇在几个 COG 功能类别中富集，但该分析没有揭示表明在人类微生物组中起作用的功能，也没有解释G. hominis在耳部感染中的发生。G. hominis基因组中不存在获得性抗菌素耐药性决定因素和毒力因子，对公开可用的 16S rRNA 基因序列和 16S rRNA 扩增子测序数据集的分析表明，G. hominis是人类皮肤微生物群的成员，可能偶尔会出现参与机会性感染。