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Performance of xylose-fermenting yeasts in oat and soybean hulls hydrolysate and improvement of ethanol production using immobilized cell systems
Biotechnology Letters ( IF 2.7 ) Pub Date : 2021-09-04 , DOI: 10.1007/s10529-021-03182-2
Paulo Roberto Dall Cortivo 1 , Luiza Fichtner Aydos 1 , Lilian Raquel Hickert 2 , Carlos Augusto Rosa 3 , Ronald E Hector 4 , Jeffrey A Mertens 4 , Marco Antônio Záchia Ayub 1
Affiliation  

We investigated the fermentation of a mixture of oat and soybean hulls (1:1) subjected to acid (AH) or enzymatic (EH) hydrolyses, with both showing high osmotic pressures (> 1200 Osm kg−1) for the production of ethanol. Yeasts of genera Spathaspora, Scheffersomyces, Sugiymaella, and Candida, most of them biodiverse Brazilian isolates and previously untested in bioprocesses, were cultivated in these hydrolysates. Spathaspora passalidarum UFMG-CM-469 showed the best ethanol production kinetics in suspended cells cultures in acid hydrolysate, under microaerobic and anaerobic conditions. This strain was immobilized in LentiKats® (polyvinyl alcohol) and cultured in AH and EH. Supplementation of hydrolysates with crude yeast extract and peptone was also performed. The highest ethanol production was obtained using hydrolysates supplemented with crude yeast extract (AH-CYE and EH-CYE) showing yields of 0.40 and 0.44 g g−1, and productivities of 0.39 and 0.29 g (L h)−1, respectively. The reuse of the immobilized cells was tested in sequential fermentations of AH-CYE, EH-CYE, and a mixture of acid and enzymatic hydrolysates (AEH-CYE) operated under batch fluidized bed, with ethanol yields ranging from 0.31 to 0.40 g g−1 and productivities from 0.14 to 0.23 g (L h)−1. These results warrant further research using Spathaspora yeasts for second-generation ethanol production.



中文翻译:

木糖发酵酵母在燕麦和大豆皮水解物中的性能以及使用固定化细胞系统提高乙醇产量

我们研究了经过酸 (AH) 或酶促 (EH) 水解的燕麦和大豆壳 (1:1) 混合物的发酵,两者都显示出高渗透压(> 1200 Osm kg -1)以生产乙醇。在这些水解产物中培养了Spathaspora、Scheffersomyces、Sugiymaella 和 Candida属的酵母,它们中的大多数是具有生物多样性的巴西分离株,并且以前未在生物过程中进行过测试。沙门孢在微需氧和厌氧条件下,UFMG-CM-469 在酸水解液中悬浮细胞培养物中显示出最佳的乙醇生产动力学。该菌株固定在 LentiKats®(聚乙烯醇)中并在 AH 和 EH 中培养。还进行了用粗酵母提取物和蛋白胨补充水解物。使用补充有粗酵母提取物(AH-CYE 和 EH-CYE)的水解物获得最高的乙醇产量,分别显示 0.40 和 0.44 g g -1 的产量以及 0.39 和 0.29 g (L h) -1 的生产率。在分批流化床下操作的 AH-CYE、EH-CYE 以及酸和酶水解产物的混合物 (AEH-CYE) 的连续发酵中测试了固定化细胞的再利用,乙醇产量范围为 0.31 至 0.40 g g-1和从 0.14 到 0.23 g (L h) -1 的生产率。这些结果保证了使用Spathaspora酵母进行第二代乙醇生产的进一步研究。

更新日期:2021-09-04
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