The aim of our study was to assess the prognostic value of the two new grading systems based on the quantification of tumor budding - TB (GBd) and poorly differentiated clusters - PDCs (PDCs-G) in colorectal carcinomas (CRC). We performed a retrospective study on 71 CRC patients who underwent surgery at the Emergency County Hospital, Timișoara. CRC cases were classified based on haematoxylin-eosin slides, using the conventional grading system, GBd and PDCs-G, respectively. We used two-tier and three-tier grading schemes for each system. Subsequently, we evaluated associations with other prognostic factors in CRC. Based on the three-tier GBd (GBd-3t) most cases (34/69, 49.27%) were classified as G3Bd-3t, while based on the conventional grading system, the majority of the cases (55/69, 79.71%) were considered G2. On the other hand, based on the three-tier PDCs-G system (PDCs-G-3t), most cases (31/69, 44.93%) were PDCs-G2-3t. We also noted a more significant association of GBd-3t with other prognostic parameters analyzed, as compared to the conventional grading system. Nodal status, tumor stage, and lymphovascular invasion were strongly correlated with GBd-3t (p=0.0001). Furthermore, we noted that PDCs-G-3t correlated more significantly than the conventional grading system with nodal status (p<0.0001), tumor stage (p=0.0003), lymphovascular invasion (p<0.0001), perineural invasion (p=0.005) and the tumor border configuration (p<0.0001). High GBd and PDCs-G grades correlate directly with other negative prognostic factors in CRC.Thus, these new parameters/classification methods could be used as additional tools for risk stratification in patients with CRC.

中文翻译：

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低分化簇和肿瘤出芽是结直肠癌的重要预后因素。

我们研究的目的是根据结直肠癌 (CRC) 中肿瘤出芽 - TB (GBd) 和低分化簇 - PDC (PDCs-G) 的量化来评估两种新分级系统的预后价值。我们对在蒂米什瓦拉县急诊医院接受手术的 71 名 CRC 患者进行了回顾性研究。CRC 病例根据苏木精-伊红载玻片进行分类，分别使用常规分级系统 GBd 和 PDCs-G。我们为每个系统使用了两层和三层分级方案。随后，我们评估了与 CRC 中其他预后因素的关联。基于三层GBd（GBd-3t），大多数病例（34/69，49.27%）被归类为G3Bd-3t，而基于常规分级系统，大多数病例（55/69，79.71%）被认为是G2。另一方面，基于三层PDCs-G系统（PDCs-G-3t），大多数情况（31/69，44.93%）为PDCs-G2-3t。与传统分级系统相比，我们还注意到 GBd-3t 与所分析的其他预后参数之间存在更显着的关联。淋巴结状态、肿瘤分期和淋巴血管侵犯与 GBd-3t 密切相关（p=0.0001）。此外，我们注意到与传统分级系统相比，PDCs-G-3t 与淋巴结状态 (p<0.0001)、肿瘤分期 (p=0.0003)、淋巴血管侵犯 (p<0.0001)、神经周围侵犯 (p=0.005) 的相关性更显着和肿瘤边界配置（p<0.0001）。高 GBd 和 PDCs-G 等级与 CRC 中的其他负面预后因素直接相关。因此，这些新的参数/分类方法可用作 CRC 患者风险分层的附加工具。