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Exogenous cortisol and red light irradiation affect reproductive parameters in the goldfish Carassius auratus
Fish Physiology and Biochemistry ( IF 2.9 ) Pub Date : 2021-09-03 , DOI: 10.1007/s10695-021-01013-9
Jin Ah Song 1 , Heung-Sik Park 2 , Young-Su Park 3 , Kang Hee Kho 4 , Cheol Young Choi 1
Affiliation  

Reproductive hormones play essential roles in the control of reproduction and gonadal maturation in fish. The purpose of this study was to determine the effects of cortisol administration (10 µg/g or 50 µg/g) or red light irradiation at two intensities (0.5 W/m2 or 1.0 W/m2) on the reproductive hormones in goldfish (Carassius auratus). The effects of different treatments were analyzed by determining the mRNA expression levels of gonadotropin-inhibitory hormone receptor (GnIH-R), chicken gonadotropin-releasing hormone (cGnRH-II), salmon GnRH (sGnRH), FSHβ, LHβ, and plasma testosterone and the level of 17β-estradiol for 48 h. Additionally, by double immunofluorescence staining, we detected the expression of both GnIH and GnRH in the diencephalons of goldfish brains. The mRNA expression of GnIH-R was significantly higher in the cortisol group and red light–irradiated group from 3 to 48 h than in the control group. Additionally, the mRNA levels of cGnRH-II, sGnRH, FSHβ, LHβ, testosterone, and 17β-estradiol were significantly lower in the cortisol group than in the other groups from 3 to 48 h. These results indicated that both cortisol and red light-emitting diode (LED) light increased GnIH expression and inhibited GnRH expression. In particular, red light irradiation suppressed reproductive responses as much as the cortisol treatment at 48 h. Thus, it could be an alternative method for suppressing reproductive responses in future aquacultures.



中文翻译:

外源皮质醇和红光照射影响金鱼鲫鱼的繁殖参数

生殖激素在控制鱼类的生殖和性腺成熟方面发挥着重要作用。本研究的目的是确定皮质醇给药(10 µg/g 或 50 µg/g)或两种强度(0.5 W/m 2或 1.0 W/m 2)的红光照射对金鱼生殖激素的影响(鲫鱼)。通过测定促性腺激素抑制激素受体(GnIH-R)、鸡促性腺激素释放激素(cGnRH-II)、鲑鱼GnRH(sGnRH)、FSHβ、LHβ和血浆睾酮的mRNA表达水平,分析不同处理的效果。 17β-雌二醇水平 48 h。此外,通过双重免疫荧光染色,我们检测到金鱼大脑间脑中 GnIH 和 GnRH 的表达。皮质醇组和红光照射组3~48 h GnIH-R mRNA表达明显高于对照组。此外,在 3 至 48 小时内,皮质醇组的 cGnRH-II、sGnRH、FSHβ、LHβ、睾酮和 17β-雌二醇的 mRNA 水平显着低于其他组。这些结果表明皮质醇和红色发光二极管 (LED) 光都增加了 GnIH 表达并抑制了 GnRH 表达。特别是,红光照射在 48 小时对生殖反应的抑制作用与皮质醇处理一样多。因此,它可能是未来水产养殖中抑制生殖反应的替代方法。

更新日期:2021-09-04
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