According to the free-radical theory of aging, accumulation of reactive oxygen species (ROS) within mitochondria throughout life span leads to impairment of the main biological macromolecules as DNA, lipids, and proteins, which might be at the basis of premature aging. One way to test experimentally such a hypothesis consists in intervention studies using antioxidant nutrients aimed at limiting or inhibiting ROS production that should be able to reduce the aging rate and disease pathogenesis. Grape seed flour (GSF) contains a high level of phytochemicals among which bioactive polyphenols exhibit numerous biological properties and beneficial health effects as antioxidant, anti-inflammatory, anticarcinogenic, multi-organ (heart, liver, kidney, and brain among others) protective. The present study aimed at testing the ability of high dosing GSF (4 g/kg bw) used as a nutritional supplement to slow down aging and prolong life span of Wistar rats when administered from early life (1-month-old animals) till their natural death. Data clearly show that high-dose GSF extends organism longevity and health span by improving multi-organ damages, systemic fueling metabolism declines, and alleviated oxidative stress and inflammation in aging rats. Our data support the extending longevity effect of grape polyphenols especially when used as high dosing nutritional supplement or as natural medicine whose appropriate galenic form as solid lipid nanoformulation is currently under investigation.

中文翻译：

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葡萄籽粉通过改善健康大鼠的多器官功能障碍和与年龄相关的氧化应激和炎症来延长寿命

根据衰老的自由基理论，线粒体内活性氧（ROS）在整个生命周期中的积累导致主要生物大分子如DNA、脂质和蛋白质的损伤，这可能是过早衰老的基础。通过实验验证这种假设的一种方法是使用抗氧化营养素进行干预研究，旨在限制或抑制活性氧的产生，这应该能够降低衰老速度和疾病发病机制。葡萄籽粉 (GSF) 含有高水平的植物化学物质，其中生物活性多酚具有许多生物特性和有益的健康作用，例如抗氧化、抗炎、抗癌、保护多器官（心脏、肝脏、肾脏和大脑等）。本研究旨在测试作为营养补充剂的高剂量 GSF（4 g/kg bw）在 Wistar 大鼠从生命早期（1 个月大的动物）直至其自然死亡。数据清楚地表明，高剂量 GSF 通过改善多器官损伤、全身燃料代谢下降以及减轻衰老大鼠的氧化应激和炎症来延长机体寿命和健康寿命。我们的数据支持葡萄多酚延长寿命的作用，特别是当用作高剂量营养补充剂或天然药物时，其适当的盖仑制剂形式作为固体脂质纳米制剂目前正在研究中。