Biobased extracts comprise various bioactive components and they are widely used in tissue engineering applications to increase bioactivity as well as physical characteristics of biomaterials. Among animal sources, garden snail Helix aspersa has come into prominence with its antibacterial and regenerative extracts and show potential in tissue regeneration. Thus, in this study, bioactive H. aspersa extracts (slime, mucus) were loaded in chitosan (CHI) matrix to fabricate porous scaffolds for hard tissue regeneration. Physical, chemical properties, antimicrobial activity was determined as well as in vitro bioactivity for bone and cartilage regeneration. Mucus and slime incorporation enhanced mechanical properties and biodegradation rate of CHI matrix. Scanning electron microscopy images showed that the average pore size of the scaffolds decreased with higher extract content. Mucus and slime extracts showed antimicrobial effect on two bacterial strains. In vitro cytotoxicity, osteogenic and chondrogenic activity of the scaffolds were evaluated with Saos-2 and SW1353 cell lines in terms of Alkaline phosphatase activity, biomineralization, GAG, COMP and hydroxyproline content. Cell viability results showed that extracts had a proliferative effect on Saos-2 and SW1353 cells when compared to the control group. Mucus and slime extract loading increased osteogenic and chondrogenic activity. Thus, the bioactive extract loaded CHI scaffolds showed potential for bone and cartilage regeneration with enhanced physical properties and in vitro bioactivity.

生物基提取物包含各种生物活性成分，它们广泛用于组织工程应用，以增加生物材料的生物活性和物理特性。在动物来源中，花园蜗牛Helix aspersa因其抗菌和再生提取物而备受瞩目，并显示出组织再生的潜力。因此，在这项研究中，将具有生物活性的H. aspersa提取物（粘液、粘液）加载到壳聚糖 (CHI) 基质中，以制造用于硬组织再生的多孔支架。物理、化学性质、抗菌活性以及体外测定骨和软骨再生的生物活性。粘液和粘液的掺入增强了 CHI 基质的机械性能和生物降解速率。扫描电子显微镜图像显示支架的平均孔径随着提取物含量的增加而减小。粘液和粘液提取物对两种细菌菌株显示出抗菌作用。体外在碱性磷酸酶活性、生物矿化、GAG、COMP 和羟脯氨酸含量方面，用 Saos-2 和 SW1353 细胞系评估支架的细胞毒性、成骨和软骨形成活性。细胞活力结果表明，与对照组相比，提取物对 Saos-2 和 SW1353 细胞具有增殖作用。粘液和粘液提取物加载增加了成骨和软骨形成活性。因此，负载生物活性提取物的 CHI 支架显示出骨和软骨再生的潜力，具有增强的物理特性和体外生物活性。